000 01727cam a2200349 a 4500
003 EG-GiCUC
005 20250223030804.0
008 130309s2012 ua h f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.03.M.Sc.2012.Ma.E
100 0 _aMarwa Mostafa Mohamed Ahmed
245 1 0 _aExperiment studies on transgenosis in chickens /
_cMarwa Mostafa Mohamed Ahmed ; Supervised Essam Abbas Elgendy , Shoukry Mohamed Eltantawy , Shawky Ahmed Mohamed
246 1 5 _aدراسات تجريبية على نقل الجينات فى الدجاج
260 _aCairo :
_bMarwa Mostafa Mohamed Ahmed ,
_c2012
300 _a71P. :
_bfacsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production
520 _aAn experiment was carried out to develop transgenic chickens through the sperm mediated gene transfer (SMGT) technique using the plasmid DNA pUC18 as a target exogene ,Chickens of two lines (CE2 and CE4) were used . Seven treatments of semen were designed and included the incubation of sperm with the plasmid with a mixture of the plasmid and lipofection at 5% or 2.5% concentration and the incubation of spermatozoa with lipofectin and a semen extender (BPSE)
530 _aIssued also as CD
653 4 _aCock spermatoza
653 4 _aLipofectin
653 4 _aSMGT
700 0 _aEssam Abbas Elgendy ,
_eSupervisor
700 0 _aShawky Ahmed Mohamed ,
_eSupervisor
700 0 _aShoukry Mohamed Eltantawy ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSoheir
_eCataloger
942 _2ddc
_cTH
999 _c41926
_d41926