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003 EG-GICUC
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008 081006s2006 ua a f m 000 0 eng d
040 _aEG-GICUC
_beng
_cEG-GICUC
041 0 _aEng
049 _aDeposite
097 _aPh.D
099 _aCai01.07.01.Ph.D.2006.Ay.B.
100 0 _aAyman EbraheemElSayed Badran
245 1 0 _aBiometrical and biotechnological aspects to evaluate some Egyptian cotton genotypes under stress conditions /
_cAyman EbraheemElSayed Badran ; Suppervised Deyaa Ahmed ElKadi , Mohamed Abd El moneam Mohamed Aly S amy Abd El Aziz Nasr Afiah
260 _aCairo :
_bAyman EbraheemElSayed Badran ,
_c2006
300 _a136P :
_bill ;
_c25cm
502 _aThesis (PH.D.) - Cairo University - Faculty Of Agriculture - Department Of Agronomy
520 _aThe genetic material used in this investigation comprised seven genotypes [Giza 83 , Giza 45 , F1 , SF2 plants , TF2 plants of the hybrid Giza 83 x Giza 45 and check varities (Giza 90 and Dendera) ] of the Egyptian cotton (Gossypium barbadense L2n=52) Giza 45 variety exhibited the least values of boll weight , Noof open bolls / plant , lint percentage , proline content and seed cotton yield / plant compared to the other genotypes under the two experiments (pots and El - Maghara region) The results indicated that agronomic and physiological characteristics were more sensitive to salinity than the technological one'sBoth SDS - PAGE of soluble proteins and Randomly Amplified Polymorphic DNA (RAPD) analysis were employed to evaluate , the genetic diversity and genetic relationships among studied genotypesFive random 10 - mer primers were used to amplify DNASimilarity matrix was generated from the RAPD analysis ; its values ranged from 0058 to 0990The salt - sensitive parent (Giza 45) and the sensitive F2 bulk , exhibited two negative PCR - RAPD markers with molecular sizes of 2489 and 1875 bp using primer OP - Z15The tolerant parent (Giza 83) and the tolerant F2 bulk exhibited three positive molecular markers with molecular sizes of 317 , 1507 and 2137 bp with OP - Z07 and OP - Z15 primersGiza 90 and Dendera (the two check varieties) seemed to be salt - tolerant genotypes as they exhibited a positive molecular marker (1507 bp) with primer Z15In conclusion , the negative markers can be used as indicators to distinguish the salt - sensitive cotton lines in breeding programs
530 _aIssued also as CD
653 4 _aBulked segregants analysis
653 4 _aGossypium barbadense L.
653 4 _aRAPD
653 4 _aSalt tolerance
653 4 _aSDS - PAGE
653 4 _aSimilarity coefficients
700 0 _aDeyaahmed ElKadi ,
_eSupervisor
700 0 _aMohamed Abd El moneam Mohamed Aly ,
_eSupervisor
700 0 _aSamy Abd El Aziz Nasr Afiah ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aEsam
_eRevisor
905 _aFayza
_eCataloger
942 _2ddc
_cTH
999 _c471
_d471