000			02231cam a2200349 a 4500
003			EG-GiCUC
005			20250223031041.0
008			140916s2014 ua h f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.10.Ph.D.2014.Re.P
100	0		_aRehab Ali Mohammed Mohammed Elhelw
245	1	0	_aProduction of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. / _cRehab Ali Mohammed Mohammed Elhelw ; Supervised Mahmoud Essam Hatem Ahmed , Mona Ibrahim Elnbaawy , Ahmed Abdelghani Elsanousi
246	1	5	_aإنتاج مجموعات إليزا علي أساس الأجسام المضادة أحادية الفسيلة لليبتوسبيرا الممرضة
260			_aCairo : _bRehab Ali Mohammed Mohammed Elhelw , _c2014
300			_a184 P. : _bfacsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aA panel of monoclonal antibodies (MAbs) against each of Leptospira interrogans serovar Canicola and pathogenic Leptospira spp. were produced. L. interrogans serovar Canicola was cultivated, concentrated and prepared for fusion. BALB/c mice of 6 weeks old were inoculated with different antigenic preparations of L. interrogans serovar Canicola with complete freund{u2019}s adjuvant. Booster doses with and without incomplete freund{u2019}s adjuvant were applied. To obtain hybridomas, mice spleen cells were fused with myeloma cells in the presence of polyethylene glycol. Hybridoma cells that secret specific antibodies were selected on the basis of ELISA. These positive hybridomas were cloned by limiting dilution method to reveal subclones of one cell origin. 5 specific clones were obtained
530			_aIssued also as CD
653		4	_aMAbs
653		4	_aPathogenic Leptospira spp.
653		4	_aSerovar Canicola
700	0		_aAhmed Abdelghani Elsanousi , _eSupervisor
700	0		_aMahmoud Essam Hatem Ahmed , _eSupervisor
700	0		_aMona Ibrahim Elnbaawy , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c47387 _d47387