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003 EG-GiCUC
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008 140917s2014 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.08.06.M.Sc.2014.Ma.M
100 0 _aMarwa Ibrahim Abdelghany Wahba
245 1 0 _aMicrobiological and biochemical studies on the production and stabilization of penicillin acylase /
_cMarwa Ibrahim Abdelghany Wahba ; Supervised Magdy Ali Amin , Ahmed Eldiwany , Magdy Elnashar
246 1 5 _aدراسات ميكروبيولوجية و بيوكيميائية لإنتاج و تثبيت إنزيم البنسيلين أسيليز
260 _aCairo :
_bMarwa Ibrahim Abdelghany Wahba ,
_c2014
300 _a274 P. :
_bcharts ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520 _aThe production of microbial penicillin G acylase (PGA) was investigated by six bacterial and two actinomycetal isolates. The PGA production by the chosen bacterial isolate, Arthrobacter viscosus NRRL B-1973, was optimized, appling statistically designed experiments. First, the Plackett-Burman design (PBD) was used to screen the effect of 19 factors, including medium components and culture conditions. Among the nineteen tested factors, only six were proven to be significant. These were; tryptone, arginine, maltose and dextrin, which exerted a significant positive effect on the PGA production, while both MgSO4 and NaCl offered significant negative effects
530 _aIssued also as CD
653 4 _aArthrobacter viscosus NRRL B-1973
653 4 _aPenicillin G acylase
653 4 _aPlackett Burman design
700 0 _aAhmed Eldiwany ,
_eSupervisor
700 0 _aMagdy Ali Amin ,
_eSupervisor
700 0 _aMagdy Elnashar ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAml
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c47395
_d47395