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003 EG-GiCUC
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008 150505s2014 ua h f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.10.10.M.Sc.2014.Aa.P
100 0 _aAalaa Samir Ahmed Saad
245 1 0 _aPrevalence and characterization of antimicrobial resistant staphylococcus species in raw meat and its zoonotic importance /
_cAalaa Samir Ahmed Saad ; Supervised kamelia Mahmoud Osman Ahmed , Aziza Mahrous Amer , Jihan M. Badr
246 1 5 _aدراسه مدى انتشار و توصيف الميكروبات العنقوديه المقاومه للمضادات الميكروبيه فى اللحوم و اهميتها الصحيه
260 _aCairo :
_bAalaa Samir Ahmed Saad ,
_c2014
300 _a222 P. :
_bfacsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aIn this study, a total number of 100 meat samples were collected from two different governorates (Giza and Kalubia) for the isolation and typing of staphylococci. Samples were obtained either from butchers shops (chicken, local beef meat) and supermarkets (imported frozen). The meat samples consisted of 50 chicken meat samples (19 breast meat, 21 thigh meat and 10 livers), 27 local beef meat and 23 imported frozen beef meat. The incidence of isolation of staphylococcus spp. was 100% from different meat samples. The majority of Staphylococcus isolates showed slime formation (40 %) and biofilm formation (85%). The prevalence of Ý- hemolysis activity of Staphylococcus spp. reached 66% while Ü- hemolysis and Þ{u2013} hemolysis activities reached 18% and 16% respectively. All Staphylococcus isolates supernatant fluid showed cytopathic effect on the vero cell line (100%). The results of antimicrobial disk diffusion test of the isolated staphylococcus spp. revealed that most of them were highly resistant to clindamycin, erythromycin, gentamicin, penicillin, sulfamethazole/trimethoprim and tetracycline (100% resistant). All the isolated Staphylococcus spp. (100 isolates) were positive for the amplification of the 16S rRNA gene (specific for genus Staphylococci) with an incidence of 100%
530 _aIssued also as CD
653 4 _aAntimicrobial resistant
653 4 _aRaw Meat
653 4 _aStaphylococcus species
700 0 _aAziza Mahrous Amer ,
_eSupervisor
700 0 _aJihan M. Badr ,
_eSupervisor
700 0 _akamelia Mahmoud Osman Ahmed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c50769
_d50769