000 02525cam a2200337 a 4500
003 EG-GiCUC
005 20250223031239.0
008 150616s2014 ua h f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.13.M.Sc.2014.Ha.I
100 0 _aHassan Ali Mohamed Fouad
245 1 0 _aIn vitro studies on propagation of some tropical fruits /
_cHassan Ali Mohamed Fouad ; Supervised Mohamed Helmy Abdelzaher , Sahar Mohamed Abdelwahab
246 1 5 _aدراسات على الإكثار المعملى لبعض الفاكهة الاستوائية
260 _aCairo :
_bHassan Ali Mohamed Fouad ,
_c2014
300 _a129 P. :
_bfacsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Pomology
520 _aThe experimental work was performed in Modern Agriculture Co. Pico Tissue Culture lab., during the period of 2012 to 2014. The aim of this work was to present a suitable protocol for propagation of date palm cv. Sewi and selected plant of the Passion fruit (Passiflora edulis) grown at "Kerdasa" region through tissue culture technique by callus. Date palm shoot tips were used with leaves primordia were sterilized by Clorox at 50% for 20 minutes and Hgcl2 at 0.1% for 10 minutes, and cultured on {u00BE} MS supplemented with 3.0 mg/l isopentyl adenine (2ip) and 2,4-D (2.5, 5.0 or 10.0 mg/l) , 1.5 g/l activated charcoal and 30 g/l sucrose (callus medium). Callus pieces (1mm3) were cultured on medium supplemented with 2,4-D at 2.0 mg/l with kin at 0.5, 1.0 or 2.0 mg/l and medium supplemented with BA at 3.0 + 2ip at 3.0 (mg/l) and 2,4- D at 20.0, 40.0 or 50.0 mg/l (the embryogenic callus medium). The embryogenic callus was cultured on medium supplemented with 0.1 mg/l NAA and 2ip at 0.1, 0.5 or 1.0 mg/l, and medium supplemented with 0.5 mg/l NAA and BA and 2ip at 0.0, 0.5 or 1.0 mg/l (the embryogenesis medium). The initiated embryos were cultured on medium supplemented with IBA at 0.0, 0.1, 0.5 and 1.0 mg/l and 0.5 mg/l BA. High concentrations of 2,4-D increased callus induction
530 _aIssued also as CD
653 4 _aDate palm
653 4 _aMicropropagation
653 4 _aPassion fruit
700 0 _aMohamed Helmy Abdelzaher ,
_eSupervisor
700 0 _aSahar Mohamed Abdelwahab ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSoheir
_eCataloger
942 _2ddc
_cTH
999 _c51340
_d51340