000			02926cam a2200349 a 4500
003			EG-GiCUC
005			20250223031352.0
008			151212s2015 ua o f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.10.M.Sc.2015.Re.M
100	0		_aReham Gamal Mohamed Abdelmagid Elrahmany
245	1	0	_aMolecular studies on some whitefly transmitted viruses affecting cucurbits / _cReham Gamal Mohamed Abdelmagid Elrahmany ; Supervised Naglaa Abdelmonem Abdullah , Haggag Salah Zein Mostafa , Ahmed Kamal Abdelsamad Elattar
246	1	5	_aدراسات جزيئية على بعض الفيروسات التى تصيب العائله القرعيه و تنقل بالذبابه البيضاء
260			_aCairo : _bReham Gamal Mohamed Abdelmagid Elrahmany , _c2015
300			_a128 P. : _bphotographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics
520			_aCucurbits are among the major vegetable crops especially in the developing countries. Cucurbits are infected with many diseases, one of the most dangerous casual agent that dramatically affect the yield production is the viral infection. In this research, two of the most important viruses affecting cucurbits, cucurbit yellow stunting disorder virus (CYSDV) and squash leaf curl virus (SLCV), were detected in many governorates in Egypt through a field survey during three successive years (2009, 2010 and 2011). The visual screening was confirmed using RT - PCR and PCR utilizing specific primers for each virus. The two viruses were molecularly and biologically characterized. The SLCV and CYSDV have been isolated and transmitted by using viruliferous whitefly to healthy cucurbits in glass cages under suitable conditions in an insect proof greenhouse. CYSDV and SLCV affect different hosts including a wide range of cucurbitacea family such as cucumber, squash, watermelon and melon as well as non - cucurbits. CYSDV symptoms were appeared on Nicotiana tabacum and chenopodium quinoa, but not appeared or transmitted to phaseolus vulgaris, Abelmoschus esculentus, Solanum lycopersicum or Morus Alba. While, SLCV infected chenopodium quinoa, pepper (capsicum annuum), eggplant (solanum melongena), phaseolus vulgaris and Nicotiana tabacum but did not infect tomato, white mulberry and okra. The CYSDV coat protein gene (CP) of the Egyptian isolate was amplified by RT - PCR then cloned and sequenced
530			_aIssued also as CD
653		4	_aCloning
653		4	_aCYSDV
653		4	_aSLCV
700	0		_aAhmed Kamal Abdelsamad Elattar , _eSupervisor
700	0		_aHaggag Salah Zein Mostafa , _eSupervisor
700	0		_aNaglaa Abdelmonem Abdullah , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c53894 _d53894