000			02994cam a2200349 a 4500
003			EG-GiCUC
005			20250223031400.0
008			151229s2015 ua do f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.10.M.Sc.2015.Mo.T
100	0		_aMona Ahmed Mohamed Abdelghani
245	1	0	_aTransient expression of ELN gene in tobacco plant / _cMona Ahmed Mohamed Abdelghani ; Supervised Naglaa Abdelmoneam Abdallah , Abdel Hadi Abdallah , Ghada Aboelheba
246	1	5	_aفى نبات الدخان ELNالتعبير المؤقت لجين ال
260			_aCairo : _bMona Ahmed Mohamed Abdelghani , _c2015
300			_a67 P. : _bcharts , photographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics
520			_aPlant molecular farming (PMF) is an important growing prospective approach in plant biotechnology. It includes production of recombinant pharmaceutical and industrial proteins in large quantities from engineered plants. Elastin is a major protein component of elastic fibers. They provide tissues with the required resilience and elasticity. In this work, Nicotiana tabacum leaves were used as a bioreactor for the expression and production of the full human tropoelastin protein; the precursor protein of elastin. The agrobacterium- mediated transient expression system into Nicotiana tabacum was used via syringe agroinfiltration to provide a fast and convenient way to produce recombinant proteins with a greater expression overall the plant leaf. This study aimed to establish an efficient and rapid system for transiently expression and production of human recombinant tropoelastin protein in transgenic N. tabacum plants. Modified sequence of the elastin (ELN) gene was biosynthesized and cloned into pCambia1390 vector to be transferred into N. tabacum. Optimization of codon usage for the human tropoelastin gene, without changing the primary structure of the protein was carried out to ensure high expression in tobacco plants. The obtained data proved that the 5th day post-infiltration is the optimum interval to obtain the maximum production of the recombinant protein. Southern blot analysis was able to detect the 2175 bps fragment representing the ELN orf. On the other hand, ELN -expression within plant{u2019}s tissue was visualized by RT-PCR within 3 to 10 days post agroinfiltration. At the protein level, western and ELISA confirmed the expression of recombinant tropoelastin protein
530			_aIssued also as CD
653		4	_aAgroinfiltration
653		4	_aMolecular farming
653		4	_aTransient expression
700	0		_aAbdelhadi Abdallah , _eSupervisor
700	0		_aGhada Aboelheba , _eSupervisor
700	0		_aNaglaa Abdelmonem Abdullah , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c54187 _d54187