000			02841cam a2200349 a 4500
003			EG-GiCUC
005			20250223031401.0
008			160102s2015 ua ho f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.10.M.Sc.2015.Me.M
100	0		_aMervat Ragab Mansour Ali Diab
245	1	0	_aMolecular genetic studies on a midgut related gene in pink bollworm - pectinophora gossypiella / _cMervat Ragab Mansour Ali Diab ; Supervised Ebtissam Hussein Aly Hussein , Samir Mohamed Mustafa Abdalla , Ahmed Mohammed Ahmed Mohammed
246	1	5	_aدراسات وراثية على جين بالمعدة الوسطى لدودة اللوز القرنفلية
260			_aCairo : _bMervat Ragab Mansour Ali Diab , _c2015
300			_a115 P. : _bfacsimiles , photographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics
520			_aVacuolar - H⁺ ATPase (V - ATPase) is a multi subunit enzyme. It consists of two domains (V₁ and V₀). V₁ domain has eight subunits (A - H) while VO domain has six subunits (a, c, c', c'', d and e). The V - ATPase is an ATP - driven proton pump and is essential to the function of eukaryotic cells. In insects, V - ATPase is located in apical membrane of goblet cells in the midgut. It acts as an energizer of the plasma membrane, driving nutrient uptake, fluid secretion and in some cases alkalizing the gut lumen. The aim of the present study was knocking down the V - ATPase gene(s) using RNAi in an attempt to disturb the food process within the pink bollworm (PBW) midgut and eventually causing the insect death. Based on the conserved regions of the V - ATPase subunits A and D genes in different insects available in the Gen Bank, degenerate primers were synthesized and used to amplify the two genes. The amplified genes encoding the V - ATPase subunits A and D transcripts were sequenced. The sequencing results confirmed the isolation of the full length genes encoding the V - ATPase subunit A was 2602 bp in length, encoding 618 amino acids. While, the gene encoding V - ATPase subunit D was 1467 bp, encoding 249 amino acids. Three dsRNA fragments were designed, two of them (VATPA756 - 1155 bp and VATPA347-753 bp) were targeting subunit A and the third - VATPD221 - 703 bp to knockdown subunit D
530			_aIssued also as CD
653		4	_aPectinophora gossypiella
653		4	_aPink bollworm
653		4	_aRNA interference
700	0		_aAhmed Mohammed Ahmed Mohammed , _eSupervisor
700	0		_aEbtissam Hussein Aly Hussein , _eSupervisor
700	0		_aSamir Mohamed Mustafa Abdalla , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c54211 _d54211