000			02899cam a2200337 a 4500
003			EG-GiCUC
005			20250223031407.0
008			160112s2015 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.08.06.M.Sc.2015.Is.C
100	0		_aIslam Mohamed Kamal Eldin
245	1	0	_aCharacterization of lactic acid bacteria from Egyptian environment and their bioactive products / _cIslam Mohamed Kamal Eldin ; Supervised Amal Emad Eldin , Tamer Mohamed Essam
246	1	5	_aتوصيف بكتيريا حمض اللبنيك من البيئة المصرية و منتجاتها الحيوية النشطة
260			_aCairo : _bIslam Mohamed Kamal Eldin , _c2015
300			_a142 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520			_aA total of 51 lactic acid bacteria (LAB) were isolated and characterized from 75 different raw food samples among which Lactobacilli represented 70% of the total isolates, followed by Lactococci 12 %, Pediococci 9 %, Leuconostocs 7 % and Streptococci 2 %. Evaluation of the probiotic and prebiotic activities were done to all isolates. Ten isolates were chosen to be acid/bile tolerant, biofilm forming, broad spectrum antimicrobial, and producing Ý-galactosidase, they also could assimilate more than 50 % of cholesterol and produce exopolysaccharides. The selected isolates were further identified by 16S rRNA gene sequencing as strains of Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus reuteri, Enterococcus faecalis, Enterococcus faecium, Pediococcus acidilactici. Screening for the sucrose metabolizing enzymes through reducing sugars production among the 10 isolates was done. Results revealed that isolates I40l Lactobacillus reuteri gave 468.5 og/ml and C7 Lactobacillus fermentum gave 57.9 og/ml reducing sugars. Comparative genomics between the 2 isolates showed that I40l Lactobacillus reuteri had sucrose phosphorylase enzyme (scrp) and levansucrase enzyme (ftfA) while C7 Lactobacillus fermentum had invertase enzyme (bfrA) which was confirmed by PCR on their specific genes (scrp) at 707 bp (ftfA) at 900 bp and (bfrA) at 479 bp respectively. The isolate I40l Lactobacillus reuteri had the ability to metabolize the anti-nutritive sugar raffinose that cause flatulence and abdominal discomfort and produced 371 og/ml reducing sugars.
530			_aIssued also as CD
653		4	_aLactic acid bacteria
653		4	_aProbiotic
653		4	_aSucrose metabolizing
700	0		_aAmal Emad Eldin , _eSupervisor
700	0		_aTamer Mohamed Essam , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c54400 _d54400