000 02294cam a2200349 a 4500
003 EG-GiCUC
005 20250223031422.0
008 160203s2015 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.10.Ph.D.2015.Eh.I
100 0 _aEhab Ali Mohamed Mohamed Fouad
245 1 0 _aImmunological studies on camels (Camelus dromedarius ) in Egypt /
_cEhab Ali Mohamed Mohamed Fouad ; Supervised Mahmoud Essam Hatem Ahmed , Jakeen Kamal Abdelhalem Eljakee , Nagwa Sayed Sayed Mohammed Ata
246 1 5 _aدراسات مناعية على الجمال وحيدة السنام فى مصر
260 _aCairo :
_bEhab Ali Mohamed Mohamed Fouad ,
_c2015
300 _a114 P. :
_bcharts ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aAs the labeled anti-camel Igs with enzymes for ELISA are unavailable in commercial level, the present investigation was directed for developing labeled anti-camel IgG with horseradish peroxidase. For purification of camel IgG whole molecule, camel sera were preliminary precipitated with 50% saturated ammonium sulphate and dialyzed against diluted PBS then concentrated. This preparation was followed by Protein A Sepharose affinity column chromatograhy for purification of camel IgG. The purity of the eluted camel IgG was tested by SDS-PAGE. Four bands of molecular weight 63, 52, 40 and 33 kDa, represent camel IgG, were detected. Anti-camel IgG was prepared by immunization of goats and rabbits separately, with purified camel IgG. Then anti-camel IgG was purified by loading on Protein A Sepharose affinity column chromatography after precipitation and concentration. Whole molecule anti-camel IgG was conjugated with horseradish peroxidase
530 _aIssued also as CD
653 4 _aCamel
653 4 _aCamelus dromedarius
653 4 _aImmunoglobulin
700 0 _aJakeen Kamal Abdelhalem Eljakee ,
_eSupervisor
700 0 _aMahmoud Essam Hatem Ahmed ,
_eSupervisor
700 0 _aNagwa Sayed Sayed Mohammed Ata ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAml
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c54904
_d54904