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| 003 | EG-GiCUC | ||
| 005 | 20250223031443.0 | ||
| 008 | 160319s2015 ua dh f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.08.06.M.Sc.2015.As.S | ||
| 100 | 0 | _aAsmaa Ahmed Abdelghaffar | |
| 245 | 1 | 0 |
_aStudy of In vitro inactivation of rabies virus by hydrogen peroxide / _cAsmaa Ahmed Abdelghaffar ;Supervised Magdy Ali Amin , Amal Emad Eldin , Abeer A. Elhalim Boseila |
| 246 | 1 | 5 | _aدراسة تثبيط فيروس داء الكلب باستخدام بيروكيد الهدروجين |
| 260 |
_aCairo : _bAsmaa Ahmed Abdelghaffar , _c2015 |
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| 300 |
_a105 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology | ||
| 520 | _aDevelopment of safe and protective vaccines against infectious pathogens remains a challenge. Inactivation of rabies virus is a critical step in the production of vaccines and other research reagents. Beta-propiolactone (ÝPL); the currently used inactivating agent for rabies virus is expensive and proved to be carcinogenic in animals. This study aimed to investigate the ability of hydrogen peroxide (H₂O₂) to irreversibly inactivate rabies virus without affecting its antigenicity and immunogenicity in pursuit of finding safe, effective and inexpensive alternative inactivating agents. H₂O₂ 3% rapidly inactivated a Vero cell adapted fixed rabies virus strain designated as FRV/K within two hours of exposure without affecting antigenicity or immunogenicity. No residual infectious virus was detected and the H₂O₂- inactivated vaccine proved to be safe and effective when compared with the same virus harvest inactivated with the classical inactivating agent (ÝPL). Mice immunized with H₂O₂-inactivated rabies virus produced sufficient level of antibodies and were protected when challenged with lethal CVS virus. These findings reinforce the idea that H₂O₂ can replace ÝPL as inactivating agent for rabies virus to reduce time and cost of inactivation process | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aHydrogen Peroxide | |
| 653 | 4 | _aInactivation | |
| 653 | 4 | _aRabies | |
| 700 | 0 |
_aAbeer A. Elhalim Boseila , _eSupervisor |
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| 700 | 0 |
_aAmal Emad Eldin , _eSupervisor |
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| 700 | 0 |
_aMagdy Ali Amin , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aSoheir _eCataloger |
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_2ddc _cTH |
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