000			03053cam a2200349 a 4500
003			EG-GiCUC
005			20250223031502.0
008			160504s2015 ua do f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.10.M.Sc.2015.Ma.B
100	0		_aMahmoud Kamel Abdelfattah Kamel
245	1	0	_aBacteriological and molecular studies on listeria monocytogenes Isolated from Rabbits / _cMahmoud Kamel Abdelfattah Kamel ; Supervised Khaled Farouk Mohamed , Sherif Abdelmonium Omar , Soad Abdelaziz Abdelwanis
246	1	5	_aدراسات بكتريولوجية و جزيئية على الليستريا مونوسيتوجينس المعزولة من الارانب
260			_aCairo : _bMahmoud Kamel Abdelfattah Kamel , _c2015
300			_a179 P. : _bcharts , photographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aListeria monocytogenes represent one of the most important pathogens that cause destructive economical losses in rabbit production, which also had gain public health concern as it can cause listeriosis in human. Due to the recent outbreaks, recalls and deaths associated with Listeria monocytogenes in rabbit farms affecting the rabbit production in Egypt. The objectives of the present study were to isolate, identificate L. monocytogenes using bacteriological examination and confirm the results by PCR and then detection of virulence genes (prfA, inlA, inlB and hlyA) in the isolated strains. One hundered samples were collected from diseased and apparently health rabbits. Out of 100 teseted samples, 32 samples were positive for L. monocytogenes by bacteriological examination, while 54 samples were positive using PCR. Where the best site for isolation of L. monocytogenes was from ear swabs (38.8%) then Liver (21.4%) but not detected in bone marrow using both techniques. Virulence gene in the 54 positive L. monocytogenes isolates were detected using selected 4 sets of primers for inlA, inlB, hylA and prfA genes, 26 samples were positive for inlA, 32 samples were positive for inlB, 31 samples were positive for hylA and 27 samples were positive for prfA by using PCR. Based on the study, PCR technique is more sensitive and rapid comparing to traditional bacteriological methods. In addition that virulence genes studies obtain accurate and specific identification and differentiation among Listeria spp. advanced studies should be applied to improve the efficiency of PCR technique for virulence study by complete gene sequence
530			_aIssued also as CD
653		4	_aIsolation
653		4	_aListeria monomcytogenes
653		4	_aRabbit
700	0		_aKhaled Farouk Mohamed , _eSupervisor
700	0		_aSherif Abdelmonium Omar , _eSupervisor
700	0		_aSoad Abdelaziz Abdelwanis , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c56257 _d56257