000 02980cam a2200349 a 4500
003 EG-GiCUC
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008 160529s2015 ua h f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.10.Ph.D.2015.Em.P
100 0 _aEman Ragab Mohammed Mostafa
245 1 0 _aPreparation of a vaccine against chlamydia psittaci /
_cEman Ragab Mohammed Mostafa ; Supervised Jakeen Kamal Abdelhaleem Eljakee , Mahmoud Dardiri Elhariri , Hadia Abdelrehim Ali
246 1 5 _aتحضير لقاح ضد الكلاميديا ستاسى
260 _aCairo :
_bEman Ragab Mohammed Mostafa ,
_c2015
300 _a156 P. :
_bfacsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aChlamydia psittaci is a biological agent endemic in nearly all bird species, thereby posing a huge zoonotic reservoir. Economic impact and financial losses resulting from avian chlamydiosis especially those in pet industries combined with that this disease is zoonotic and transmissible to human highlight the necessity of application of proper control measures. In the present study the potential risk of Chlamydia psittaci infection was determined by isolation of chlamydia psittaci in excreta and conjunctival samples of two different genera of budgerigars including melopsittacus undulatus and Agapornis pullarius. The prevalence of chlamydiosis using Gimenez stain (70.3% in australian budgerier, 52.9%in English budgerier, 66.7% in red faced pied lovebird) and direct molecular identification (45.9% in australian budgerier, 64.70 %in English budgerier, 57.1% in red-faced pied lovebird) was high, that suggests that pet bird owners could be under the risk of chlamydial infection. For the first time in Egypt, in the present work, we tried to prepare inactivated vaccine against chlamydia psittaci. Embryonated egg grown material of chlamydia psittaci strains was used as a whole bacterin to vaccinate chickens as available model to avian species. In the Immunization experiment, chlamydia shedding was evaluated through cloacal swabs inoculation in embryonated chicken eggs as well as humoral immune response was evaluated using microimmunoflourescence test. The immunological trial revealed that killed vaccine can effectively reduce chlamydial shedding from birds with proper level of protective antibody titer which made an effective barrier for protection
530 _aIssued also as CD
653 4 _aChlamydia psittaci
653 4 _aPet industries
653 4 _aVaccine microimmunoflourescence
700 0 _aHadia Abdelrehim Ali ,
_eSupervisor
700 0 _aJakeen Kamal Abdelhaleem Eljakee ,
_eSupervisor
700 0 _aMahmoud Dardiri Elhariri ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c56684
_d56684