| 000 | 02622cam a2200337 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223031516.0 | ||
| 008 | 160529s2015 ua h f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
||
| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.10.10.Ph.D.2015.Sh.I | ||
| 100 | 0 | _aShaymaa Abdelmalek Mohammed | |
| 245 | 1 | 0 |
_aImmunological studies on mycobacterium avium subsp. paratuberculosis for improving the immunodiagnosisof Johne'sdisease / _cShaymaa Abdelmalek Mohammed ; Supervised Rafik Tawfik Mohammed Soliman , Khaled Farouk Mohammed Alamry |
| 246 | 1 | 5 | _aدراسات مناعيةعلى ميكروب المايكوباكتيريام أفيام تحت نوع باراتيوبركيولوزيس لتطويرالتشخيص المعملى لمرض يونز |
| 260 |
_aCairo : _bShaymaa Abdelmalek Mohammed , _c2015 |
||
| 300 |
_a149 P. : _bfacsimiles ; _c25cm |
||
| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology | ||
| 520 | _aMycobacterium avium subsp. paratuberculosis (MAP) is the cause of Johne's disease (JD), a chronic granulomatous intestinal disease of cattle. The disease represents one of the important problemsfacing dairy industry andcausing high economic losses. 100 fecal and 100serum samples were collected from JD clinically suspected cases.Sixtyout of the 100 fecal samples werepositive usingZiehl Neelsen stain showing acid and alcohol fast red bacilli.. Bacteriological examination of the 100 fecal samples reveled the isolation of 34MAP isolates.The recovered MAPisolates were tested by conventional and nested PCR.The nested PCR provedthe identity of the 34 MAP isolates; however, the conventional PCR using different primers gave variable results. Using a commercialMAP specific ELISAkits, only 13 out of the 100 serum samples were positive. The recoveredMAP isolates were cultured on modified Middlebrook7H9 and modified Watson Reid liquid media for preparation of theMAP cultural filtrate (CF) proteins, which were then separated by SDS-PAGE.The MAPCF immunogenic determinants were identified using western-Blot technique. Different immunogenic bands from 9-72 kDa were identified | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aImmunodiagnosis | |
| 653 | 4 | _aMycobacterium avium subsp | |
| 653 | 4 | _aParatuberculosis | |
| 700 | 0 |
_aKhaled Farouk Mohamed Alamry , _eSupervisor |
|
| 700 | 0 |
_aRafik Tawfik Mohamed Soliman , _eSupervisor |
|
| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aAml _eCataloger |
||
| 905 |
_aNazla _eRevisor |
||
| 942 |
_2ddc _cTH |
||
| 999 |
_c56709 _d56709 |
||