000			02917cam a2200349 a 4500
003			EG-GiCUC
005			20250223031635.0
008			170104s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.21.M.Sc.2016.Ha.P
100	0		_aHadeer Hesham Abdelfatah
245	1	0	_aPhenotyping and cytokines profiling of myeloid dendritic cells in breast cancer patients / _cHadeer Hesham Abdelfatah ; Supervised Somaya Osman Eldeeb , Mona Mostafa Mohamed , Mohamed Elsayed Elshinawi
246	1	5	_aالتنميط المظهرى وتعريف السيتوكينات للخلايا الشجيرية النخاعية فى مرضى سرطان الثدى
260			_aCairo : _bHadeer Hesham Abdelfatah , _c2016
300			_a184 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520			_aDendritic cells (DCs) are the most potent antigen presenting cells (APCs) that have the capability to encounter tumor antigens and migrate to lymph nodes (LNs) to complete their maturation. Tumor growth factor-beta (TGF-Ý) as one of tumor derived factors was shown to play a major role in controlling DCs function. The aim of the present study was to assess the mutual interaction between myeloid DCs (mDCs) and breast cancer cells. Numbers of CD11c+ myeloid DCs (mDCs) were counted in peripheral and axillary tributaries blood of breast cancer patients, isolated by negative selection. Culture media containing mDCs derived factors were used to stimulate breast cancer cell line MDA-MB-231 and MCF-7 utilizing 3D in-vitro tissue culture models. Cytokines profiling of mDCs was done using cytokine antibody array. The results showed reduction in secretory function and count of mDCs. TGF-Ý was more expressed in Breast carcinoma tissue of patients with positive LN metastasis with lower infiltration of CD11c+ cells than negative LN metastasis. Moreover, TGF-Ý treated mDCs was observed with elevated levels of apoptotic mDCs even after LPS stimulation. Axillary-tributaries mDCs induce increase in colony size of breast cancer cell lines. In conclusion, the presented results suggest impairment in breast cancer patient's mDCs function with suggesting TGF-Ý to be a major responsible for mDCs dysfunction. Blocking of TGF-Ý emerge as therapeutic target in breast cancer patients with positive LN metastasis
530			_aIssued also as CD
653		4	_aAxillary-tributaries
653		4	_aBreast cancercells
653		4	_aTGF-Ý
700	0		_aMohamed Elsayed Elshinawi , _eSupervisor
700	0		_aMona Mostafa Mohamed , _eSupervisor
700	0		_aSomaya Osman Eldeeb , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSoheir _eCataloger
942			_2ddc _cTH
999			_c59325 _d59325