| 000 | 03183cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223031657.0 | ||
| 008 | 170304s2016 ua ho f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
||
| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.08.06.M.Sc.2016.He.S | ||
| 100 | 0 | _aHeba Attia Mohammed Attia | |
| 245 | 1 | 0 |
_aStudies on NDM-1 like genes in local gram negative isolates / _cHeba Attia Mohammed Attia ; Supervised Alaa Eldin Mahmoud shawky , Amal Emad Eldin , Aymen Samir Yassin |
| 246 | 1 | 5 | _aأو {uئإإ٣}{uئإ٨إ} {uئآئإ}{uئإآ٨}{uئإ٨إ}{uئإ٩١}{uئآءؤ}{uئآءآ} {uئإؤ٣}{uئإئ٠} {uئإأآ}{uئإآ٠}{uئإئآ}ت {uئإإ٣}{uئإء٤}{uئإإ٠}{uئآئئ}{uئإ٩٤} {uئإآ٣}{uئإ٨إ}{uئإؤئ}{uئإ٩٢}{uئإ٩٤} ا{uئإؤئ}{uئإء٠}{uئإءإ}ام NDM-1 درا{uئإآ٣}{uئإ٨إ}ت {uئإء٣}{uئإإإ}ل {uئإ٩ئ}{uئآئئ}{uئإإ٦} ال |
| 260 |
_aCairo : _bHeba Attia Mohammed Attia , _c2016 |
||
| 300 |
_a225 P. : _bfacsimiles , photographs ; _c25cm |
||
| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology | ||
| 520 | _aNew delhi metallo-Ý-lactamase (NDM-1) is a recently discovered novel plasmid-borne MBL enzyme, capable of hydrolyzing a broad range of antibiotics including the carbapenem antibiotics that are the mainstay for the treatment of antibiotic-resistant bacterial infections. The goal of this work was to detect the presence of genes coding for this enzyme in local isolates from some Egyptian health settings. A total of 235 Gram-negative clinical isolates recovered from different sites of infections were collected and identified by proper microscopic and biochemical methods. Susceptibilities of all isolates were tested against 23 antimicrobials of different classes using disc diffusion technique, where 63 isolates of 235 total isolates were shown to be multi-drug resistant. Multi-drug resistant isolates were tested for the presence of the ESBLs and for the presence of MBLs and othere carpabenamases by MIC determination against specific antibiotics as well as several phenotypic tests. ERIC typing was used to classify the isolates into related groups.Polymerase chain reaction was used to confirm the presence of bla NDM-1 gene in the phenotypically positive isolates using a set of species-specific in addition to a novel universal primer. Sequencing was done on several isolates and the BLAST results showed high similarities to bla NDM-1 sequences previously identified in pakistan and cuba suggesting the origin of the isolates. Antibiotic resistance will always represent a major challenge to health care policies worldwide | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aGram negative bacteria | |
| 653 | 4 | _aMetallo Ý-lactamases | |
| 653 | 4 | _aMulti drug resistance | |
| 700 | 0 |
_aAlaa Eldin Mahmoud shawky , _eSupervisor |
|
| 700 | 0 |
_aAmal Emad Eldin , _eSupervisor |
|
| 700 | 0 |
_aAymen Samir Yassin , _eSupervisor |
|
| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
||
| 905 |
_aSamia _eCataloger |
||
| 942 |
_2ddc _cTH |
||
| 999 |
_c60079 _d60079 |
||