| 000 | 02708cam a2200337 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223031727.0 | ||
| 008 | 170601s2016 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
||
| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.10.03.M.Sc.2016.Mo.S | ||
| 100 | 0 | _aMohamed Adel Mohamed AboElfetouh Mohamed Elbehairy | |
| 245 | 1 | 0 |
_aStudies on Newcastle disease in poultry flocks in Egypt / _cMohamed Adel Mohamed Abo Elfetouh M. Elbehairy ; Supervised Mostafa Ahmed Bastami , Manal Afifi Ali |
| 246 | 1 | 5 | _aدراسات عن مرض النيوكاسل فى مزارع الدواجن فى مصر |
| 260 |
_aCairo : _bMohamed Adel Mohamed AboElfetouh Mohamed Elbehairy , _c2016 |
||
| 300 |
_a161 P. : _bcharts , facsimiles ; _c25cm |
||
| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Animal Diseases | ||
| 520 | _aSeventy four chicken, turkey and pigeon flocks were investigated for the presence of NDV using RT-PCR F-protein gene assay, 26 flocks tested positive for NDV. As a preliminary step for the exclusion of lentogenic vaccinal strain positive RTPCR samples were tested by real time reverse transcriptase polymerase chain reaction (rRTPCR) using a probe specific to velogenic strains. Nineteen samples tested positive for vNDV while seven samples tested negative. Twelve samples out of the positive samples were isolated on SPF ECE from eight different Egyptian governorates. Partial sequencing of the F-Protein gene revealed that all isolated strain have multiple basic A.A at the F-Protein cleavage site possessing the velogenic motif (112.RRQKR*F.117). Phylogenetic analysis of the F-protein 375 b.p. hypervariable region (nt 47{u2013} 422), revealed that all isolates are belonging to genotype VIId with (99%-100%) amino acid identity matrix with the Israelian (Turkey-Israel-111-2011) and Chinese (Chicken-China-SDYT03-2011) strain, Genotype VII. Single pure NDV isolated strain was selected APMV1/chicken/EG-BH/POD.CU/2015 (EG.BH/2015) after testing negative for AI common gene, IB, and IBD virus. Titration of the selected strain reveals MDT of (44 hr.) and 107.5 ELD50/ 0.1 ml. In vivo vaccination protection study was done using different vaccination programs for protection against challenge with the locally isolated (EG.BH/2015) | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aEgypt | |
| 653 | 4 | _aGentype VIId | |
| 653 | 4 | _aVelogenic NDV | |
| 700 | 0 |
_aManal Afifi Ali , _eSupervisor |
|
| 700 | 0 |
_aMostafa Ahmed Bastami , _eSupervisor |
|
| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aEnas _eCataloger |
||
| 905 |
_aNazla _eRevisor |
||
| 942 |
_2ddc _cTH |
||
| 999 |
_c61043 _d61043 |
||