000			03014cam a2200349 a 4500
003			EG-GiCUC
005			20250223031728.0
008			170604s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.07.Ph.D.2016.Az.U
100	0		_aAzza Moustaffa Moustaffa Abdelmoteleb
245	1	0	_aUse of the recent technology in identification and detection of meat adulteration in some animals / _cAzza Moustaffa Moustaffa Abdelmoteleb ; Supervised Abdelazeim A. Ahmed , Eiman M. Elsaied , Marwa I. Abdelhamied
246	1	5	_aاستخدام التقنيه الحديثه للاستعراف و الكشف عن غش اللحوم لبعض الحيوانات
260			_aCairo : _bAzza Moustaffa Moustaffa Abdelmoteleb , _c2016
300			_a97 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Forensic Medicine and Toxicology
520			_aThis study aimed to evaluate the performances precipitation test (a standard protocol applied in Egypt on batches or raw material and finished beef products) for identification of animal species in experimental mixture of fresh minced cattle meat with minced meat of camel, donkey, pig and dog meat with known formulations. In addition, conventional PCR procedure was utilized for detecting different animal species in experimental freshly minced meat samples. Precipitation technique was performed using specific antiserum, which was prepared previously; when the precipitate was formed, it considered a positive result. PCR technique was performed for 35 cycles. The amplified DNA fragments were run on agarose gel stained and visualized using a UV transilluminator. The present result revealed that, precipitation technique was successfully used for identification of individual meat species and detection of adulteration of cattle meat by another one species and low effective in identification of more than three species in freshly minced meat mixture. Agarose gel analysis of PCR product amplified with species specific primers showed that mitochondrial DNA fragments of cattle, camel, donkey, pig and dog meats were 271, 208, 439, 212 and 322 base pair after an amplification of 35 cycles. PCR method could detect animal meat species in freshly minced meat mixtures of two, three species and more than three species. On the other side, PCR could not identify of camel in meat mixture of four species in presence of donkey meat and in meat mixture contain five different species
530			_aIssued also as CD
653		4	_aIdentification
653		4	_aMeat
653		4	_aPrecipitation test
700	0		_aAbdElazeim Ali Ahmed , _eSupervisor
700	0		_aEiman Mostafa Elsaied , _eSupervisor
700	0		_aMarwa Ibrahim AbdElhamied , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c61073 _d61073