000 02442cam a2200349 a 4500
003 EG-GiCUC
005 20250223031802.0
008 170907s2016 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.10.Ph.D.2016.Mo.B
100 0 _aMohamed Ahmed Gamal Abdelaziz
245 1 0 _aBacteriological and molecular studies on Bacillus cereus isolated from meat and meat products /
_cMohamed Ahmed Gamal Abdelaziz ; Supervised Khaled Farouk Mohamed Alamry , Mahmoud Dardiri Elhariri , Hussein Mohamed Hussein
246 1 5 _aدراسات بكتريولوجية و جزيئية علي الباسيلاس سيريز المعزوله من اللحوم و منتجاتها
260 _aCairo :
_bMohamed Ahmed Gamal Abdelaziz ,
_c2016
300 _a153 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aRandomly, 150 different meat &meat products and chicken products samples [15 rice kofta, 15 oriental sausage, 20 raw meat, 20 minced meat, 20 meat lunchon, 20 chicken pane, 20 chicken nuggets and 20 chicken burger] were collected from super markets and local markets in Cairo, Giza, and Alexandria Governorates, Egypt. Morphological, biochemical and molecular identification of bacterial isolates revealed B.cereus with total incidence of 18.66%. While the individual incidence was 15% in raw meat, 20% in minced meat, 20% in meat lunchon, 35% in chicken panee, 25% in chicken nuggets, 25% in chicken burger and 0% in both oriental sausage and rice kofta. B.cereus harbour toxin genes (hbl, nhe, cyt K and ces). Carvacrol was successfully used at different concentrations (0.02 ml/ml, 0.04 ml/ml and 0.06 ml/ml) to decrease the toxin genes transcription. SYBR Green real time PCR proved that the four toxin genes was functioning and undergoing transcription, besides its ability to monitor the influence of carvacrol usage
530 _aIssued also as CD
653 4 _aB.cereus
653 4 _aChicken products
653 4 _aMeat
700 0 _aHussein Mohamed Hussein ,
_eSupervisor
700 0 _aKhaled Farouk Mohamed Alamry ,
_eSupervisor
700 0 _aMahmoud Dardiri Elhariri ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aEnas
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c62208
_d62208