000			02940cam a2200349 a 4500
003			EG-GiCUC
005			20250223031957.0
008			180417s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2017.Mo.P
100	0		_aMohamed Salah Sayed
245	1	0	_aProduction of some bioactive compound(s) from Aspergillus spp. / _cMohamed Salah Sayed ; Supervised Mohamed Ibrahem A. Ali , Ahmed Hussien Abdelfatah , Neveen Mahmoud Khalil
246	1	5	_aإنتاج بعض المركبات النشطة حيويا من فطر الأسبرجيللس
260			_aCairo : _bMohamed Salah Sayed , _c2017
300			_a144 P. : _bcharts , facsimils ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology
520			_aTwenty five Aspergillus isolates were screened from Giza Governorate and Saint Catherine Protectorate soils in Egypt. The antimicrobial activity of the crude extracts was tested against two Gram positive bacteria (Bacillus subtilis NRRL-B-4219, Staphylococcus aureus ATCC29213), four Gram negative bacteria (Alcaligenes faecalis B-170, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC10131, Pseudomonas aeruginosa ATCC27953), and one yeast (Candida albicans ATCC10231). The antioxidant activity using free radical scavenging model was assayed for the crude extracts. The anticancer activity for all of crude extracts was determined against HCT116 (Colon carcinoma cell line), HEPG2 (Liver carcinoma cell line), and MCF-7 (Breast carcinoma cell line). Aspergillus nomius was the most potent fungal species accordingly, it was chosen for bioactivity assay. Identification of this species was further confirmed at the molecular level based on nuclear ribosomal DNA 18s identities. An accession number, LC199488, was given at the DDBJ GenBank. The column chromatography of its crude extract yielded five distinguished fractions. The biological (antimicrobial, antioxidant and antitumor) activities of these fractions were assayed. Fraction B proved to be of most potential. HPLC analysis of this fraction showed that there was a sharp and clear peak at about 18.1 min. This denoted the presence of an active compound. The compound at this peak was purified and its structure was elucidated via ¹ HNMR and ¹³ CNMR spectroscopy. It was concluded that it would be 1,2,3,9 tetrahydropyrrolo [2,1-b] quinazolin-3-ol
530			_aIssued also as CD
653		4	_aAspergillus spp.
653		4	_aHPLC
653		4	_aProduction of some bioactive compound(s)
700	0		_aAhmed Hussien Abdelfatah , _eSupervisor
700	0		_aMohamed Ibrahem A. Ali , _eSupervisor
700	0		_aNeveen Mahmoud Khalil , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c65913 _d65913