000 02696cam a2200337 a 4500
003 EG-GiCUC
005 20250223032000.0
008 180428s2016 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.05.M.Sc.2016.Sa.R
100 0 _aSara Mohamed Khalid Elshafie
245 1 0 _aResponse of diabetic feets{u06E5}pathogenic bacteria to heavy metal salts /
_cSara Mohamed Khalid Elshafie ; Supervised Mohamed Ez Eldin Abdelhaleem Dawoud , Ibrahim Ali Emara
246 1 5 _aإستجابة البكتريا الممرضة في أقدام مرضى السكرى لأملاح المعادن الثقيلة
260 _aCairo :
_bSara Mohamed Khalid Elshafie ,
_c2016
300 _a71 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology
520 _aThis investigation aimed at the study of the effect of heavy metals salts solutions on the pathogenic bacteria isolated from diabetic Feet's patients in early stages. Isolation and identification of isolated bacteria based on biochemical, morphological and molecular biology techniques (PCR using 16S rRNA primer). In this experiment, 27bacteria was isolated and identified; it included 8 Escherichia coli, 7 Staphylococcus spp., 5 Pseudomonas spp., 4 klebsiella spp. and 3 Proteus spp. Isolates was confirmed by 16S rRNA gene sequence analysis. Also these bacteria tested for susceptibility to commonly used antibiotics and found that mainly meropenem , and cephoperazone +sulbactam were the most effective antibiotics against bacteria. On studying the physiology and growth bactericidal response of these bacteria to different concentration of some selected heavy metal salts (e.g. Copper & Zinc) solutions with different concentrations (10 oM{u2013} 10⁷ oM), Revealed that the most effective concentration of heavy metals salt solutions CuSO4 & ZnSO4 are (10⁵ oM). These solutions were tested on the tissue of patients to make sure that these concentrations were nontoxic. After detection of the nontoxic& most inhibitory concentration to different pathogenic bacteria. These obtained results can be applied for treatment of diabetic feet's pathogenic bacteria as a lotion
530 _aIssued also as CD
653 4 _aDiabetic feet
653 4 _aHeavy metals
653 4 _aPathogenic bacteria
700 0 _aIbrahim Ali Emara ,
_eSupervisor
700 0 _aMohamed Ez Eldin Abdelhaleem Dawoud ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c66014
_d66014