000			03174cam a2200349 a 4500
003			EG-GiCUC
005			20250223032043.0
008			180913s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.08.06.M.Sc.2018.Sa.I
100	0		_aSalma Hussein Abdelfattah
245	1	0	_aInvestigating the distribution and potential function of members of the Mmp-protein superfamily in escherichia coli / _cSalma Hussein Abdelfattah ; Supervised Mohamed Abdelaziz Toema , Ramy Karam Aziz , Reham Samir Mohamed
246	1	5	_aو دراسة وظائفها المحتملة Escherichia coli فى بكتيريا الإشيريشيا القولونية MmpL استقصاء توزيع بروتينات عائلة
260			_aCairo : _bSalma Hussein Abdelfattah , _c2018
300			_a150 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520			_aEscherichia coli is a beneficial common resident of the intestine, but a few strains can be pathogenic, e.g.E. coli O157:H7, which causes serious diseases. Because of E. coli{u2019}s versatility and genome plasticity, the bacteria have alarmingly developed resistance against many commonly used antibiotics by different mechanisms. Although E. coli has been extensively studied, around 25% of its genes remain unknown. Thus, it is possible to discover novel resistance genes. One resistance mechanism is the acquisition of genes encoding efflux pumps, among which are the mycobacterial membrane proteins (Mmp), classified as resistance, nodulation and division (RND) efflux pumps. In Mycobacteria, these proteins are involved in the transport of mycolic acid precursors outside the cell wall. In methicillin-resistant S. aureus, an MmpL homolog is involved in oxadiazole resistance. In E. coli, members of this family were characterized in commensal strains as coli K-12, but not in pathogenic ones. In this study we investigated the distribution of mmpL genes (z4861 and yegN) found in E. coli O157:H7 and their potential function by designing knockout mutants and studying the impact of their deletion. Both bioinformatics analysis and PCR screening of clinical isolates demonstrated that yegN is more abundant than z4861. All mutants, when compared to the WT strain, did not show any change in their growth pattern under either aerobic or anaerobic condition. The three mutant strains showed significant increased susceptibility to fluoroquinolones than the WT strain, and this finding was further corroborated by transcriptional analysis that showed a significant increase in z4861 and yegN RNA levels when subjected to ciprofloxacin
530			_aIssued also as CD
653		4	_aE. coli
653		4	_aEfflux pump
653		4	_aResistance
700	0		_aMohamed Abdelaziz Toema , _eSupervisor
700	0		_aRamy Karam Aziz , _eSupervisor
700	0		_aReham Samir Mohamed , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c67454 _d67454