000 02828cam a2200349 a 4500
003 EG-GiCUC
005 20250223032049.0
008 180925s2017 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.12.M.Sc.2017.Ho.P
100 0 _aHossam Abdelmohsen Abdelaty
245 1 0 _aPropagation of Hydrangea by tissue culture technique /
_cHossam Abdelmohsen Abdelaty ; Supervised Azza Mohamed Saed Arafa , Samia Sami Helme , Ahmed Abbas Nower
246 1 5 _aاكثار نبات الهيدرانجيا باستخدام تقنية زراعة الانسجة
260 _aCairo :
_bHossam Abdelmohsen Abdelaty ,
_c2017
300 _a102 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Ornamental Horticulture
520 _aHydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. The goal of this research was to set a protocol for in vitro propagation of Hydrangea macrophylla for commercial production and develop efficient system for synthetic seed production and storage of Hydrangea macrophylla, for this purpose two type of explant (shoot tip and node) were sterilized and the best percentage of survival explants (40%) was obtained when explants immersed for 20 min in 1 or 1.5 % NaOCl. For in vitro multiplication, Murashige and Skoog medium supplemented with 1.0 mg/l BA and 2.0 mg/l Kin was the best treatment on shoot number. Using GA3 at 1.0 mg/l was a marked increase in plant length when compared with zero level (control). During the rooting stage, the medium containing 4.0 mg/l IAA with charcoal gave the longest shoot and the highest number of leaves, while NAA at 1.0 mg/l without charcoal gave the highest number of roots and the longest root was found at 2.0 mg/l IAA without charcoal. The longest plant ( 6.17 cm) was achieved when peat moss : perlite ( 2 : 1 v/v ) acclimatization mixture was used compared with other mixtures. Hydroponic acclimatization system gave higher means (18.83 cm2) and (36.88) for leaf area and total chlorophyll respectively. Shoot tips encapsulated with 4% alginate and hardened in 100 mM CaCl₂.2H₂O and it was found best composition for gel complexation, which produces firm, clear, and isodiametric beads
530 _aIssued also as CD
653 4 _aBA
653 4 _aHydrangea macrophylla
653 4 _aIn vitro
700 0 _aAhmed Abbas Nower ,
_eSupervisor
700 0 _aAzza Mohamed Saed Arafa ,
_eSupervisor
700 0 _aSamia Sami Helme ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c67653
_d67653