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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.24.03.Ph.D.2018.Ma.L
100 0 _aMahmoud Esmail Mohamed Alalfy
245 1 0 _aLaser based detection of maternal plasma cell free fetal DNA for early fetal sex determination /
_cMahmoud Esmail Mohamed Alalfy ; Supervised Mohamed Amr Hussein Elnoury , Osama Mahmoud Azmy , Jehan Mohamed Alsharnoubi
246 1 5 _aالتحديد المبكرلجنس الجنين بإستخدام تقنيه الليزر للحامض النووى الجنينى الحر الموجود فى بلازما دم الأم
260 _aCairo :
_bMahmoud Esmail Mohamed Alalfy ,
_c2018
300 _a173 P. :
_bcharts ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - National Institute of Laser - Department of Laser Application in Medical and Biological
520 _aEnhancement of DNA detection assays increases the use of non-invasive prenatal diagnosis that consequently enhances the supervision plan of pregnant woman with genetic disorder that could have an expected babies with this disorder. Aim of work: current work was planned to examine plasma of pregnant women starting from 6 th week of the pregnancy for Cell-free fetal DNA, to determine embryonic sex type with consequent prospect of detection of fetal anomalies such as sex-linked genetic abnormalities . Method: samples collected from 6 th week till 11 th week of pregnancy according to pregnancy age and tested using Real time PCR for determination of fetal sex Results: testing of samples resulted in detection of fetal sex starting from 6th week and the later the gestational age ,the better the result for detection of fetal sex . all results were confirmed by Ultrasound scan and neonatal outcome. Testing results revealed PCR detection for 58 males and 92 females with confusion in one fetus due to non identical twins
530 _aIssued also as CD
653 4 _aDNA
653 4 _aMaternal plasma
653 4 _aSex determination
700 0 _aJehan Mohamed Alsharnoubi ,
_eSupervised
700 0 _aMohamed Amr Hussein Elnoury ,
_eSupervised
700 0 _aOsama Mahmoud Azmy ,
_eSupervised
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c68619
_d68619