000 03120cam a2200349 a 4500
003 EG-GiCUC
005 20250223032144.0
008 190105s2018 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.08.07.M.Sc.2018.Ma.C
100 0 _aMahmoud Anter Mohamed Arafat
245 1 0 _aComparative pharmacognostical study and tissue culture of sequoia sempervirens (D. Don Endl) and taxodium distichum (L. Rich) cultivated in Egypt /
_cMahmoud Anter Mohamed Arafat ; Supervised Seham Salah Eldin Elhawary , Mohamed Abdelatty Rabeh , Essam M. Abdelkadder
246 1 5 _aدراسة عقاقيريه مقارنة وزراعة الأنسجة لنباتى السيكويا سيمبيرفيرينز (د.دون إندل) والتاكسوديم ديستيكم (ل.ريتش) المزروعين فى مصر
260 _aCairo :
_bMahmoud Anter Mohamed Arafat ,
_c2018
300 _a230 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy
520 _aAiming to identify and authenticate Sequoia sempervirens (D.Don Endl) and Taxodium distichum (L.Rich) cultivated in Egypt. RAPD technique was employed to identify unique DNA markers and establish a typical fingerprint for S. sempervirens and T. distichum. The amplification profile of S. sempervirens was screened and a total of 18 different RAPD fragments had been recorded while the amplification profile of T. distichum was screened and a total of 15 different RAPD fragments had been recorded. Macromorphological and micromorphological studies of S. sempervirens and T. distichum had been done. The results of preliminary phytochemical screening of both plants showed the absence of alkaloids, anthraquinones, catechol tannins or cardiac glycosides in non-flowering parts of S. sempervirens and T. distichum. Sterols and/or triterpenoids, pyrogallol tannins, carbohydrate and/or glycosides and flavonoids were detected in all of the tested parts of the plants. Volatile substances were detected in very low amounts in non-flowering parts of both plants. GC/MS analysis of hexane extract of non-flowering parts of S. sempervirens and T. distichum revealed that unsaturated fatty acids were present in higher percentage than saturated fatty acids in both S. sempervirens and T. distichum. 8?-hydroxypimar-15-en-19-oic acid had been isolated from hexane fraction of T. distichum. Apigenin, luteolin and tricetin had been isolated from ethyl acetate fraction of S. sempervirens and hyperoside and catechin had been from ethyl acetate fraction of T. distichum
530 _aIssued also as CD
653 4 _aGenetic profiling
653 4 _aSequoia sempervirens
653 4 _aTaxodium distichum
700 0 _aEssam M. Abdelkadder ,
_eSupervisor
700 0 _aEssam M. Abdelkadder ,
_eSupervisor
700 0 _aMohamed Abdelatty Rabeh ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c69430
_d69430