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003 EG-GiCUC
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008 190316s2018 ua do f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.06.M.Sc.2018.Mo.B
100 0 _aMoustafa Mohamed Abdelhady Zohair
245 1 0 _aBiochemical studies on bioactive products from soil fungi /
_cMoustafa Mohamed Abdelhady Zohair ; Supervised Mahmoud Wafik Sadik , Mohammed Zakeria Sedik , Eman Ramadan Hamed
246 1 5 _aدراسات كيميائية حيوية على المنتجات الفعالة المستخرجة من فطريات التربة
260 _aCairo :
_bMoustafa Mohamed Abdelhady Zohair ,
_c2018
300 _a166 P. :
_bcharts , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology
520 _aThe objectives of this thesis were to isolate, screen and select the most active fungal isolates from the rhizosphere of the medicinal plants Basil, (Ocimum basilicum), peppermint (Mentha piperita) and (Aloe vera) and evaluate their efficacy to introduce a promising solution in biological control applications. Also using agro-industrial wastes as solid support, carbon source in solid-state fermentation processes for the production of value-added compounds such as antioxidant and antimicrobial agents. Among the collection of 104 fungal isolates, 59 of them were assessed for in vitro antagonistic activity against phytopathogenic fungi; Fusarium solani, Rhizocotina solani, Sclerotium rolfsii and Verticillium dahliae. The most active antagonistic isolates were identified using molecular tools based on 18S rDNA. The sequence data of Aspergillus pseudocaelatus and Trichoderma gamsii have been submitted to GenBank given the accession No. MG772677 and KX685665, respectively. The antagonistic mechanisms were evaluated using confrontation method and scanning electron microscopy. Chemical investigation of extracts of A. pseudocaelatus, T. gamsii strains and mixed culture of them was analyzed using GC/MS analysis of volatile organic compounds (VOCs), preliminary metabolomic profile analysis by LC/MS. Biological investigation on the ethyl acetate extract of A. pseudocaelatus MG772677 and T. gamsii KX685665 strains was evaluated. The greenhouse application with the selected isolates on basil seedlings was performed
530 _aIssued also as CD
653 4 _aAspergillus pseudocaelatus
653 4 _aMycoparasitism
653 4 _aTrichoderma gamsii
700 0 _aEman Ramadan Hamed ,
_eSupervisor
700 0 _aMahmoud Wafik Sadik ,
_eSupervisor
700 0 _aMohammed Zakeria Sedik ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c70854
_d70854