000 02913cam a2200349 a 4500
003 EG-GiCUC
005 20250223032257.0
008 190508s2018 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.07.13.M.Sc.2018.Ma.I
100 0 _aMaha Rashed Ahmed Alsayed
245 1 0 _aImproving productivity of somatic embryogeneis from immature inflorescence of date palm in vitro /
_c Maha Rashed Ahmed Alsayed ; Supervised Mohamed Ahmed Fayek , Mohamed Helmy Abdelzaher , Eman Mohamed Mostafa Zayed
246 1 5 _aتحسين إنتاجية الآجنه الجسميه الناتجه من البراعم الزهريه غير البالغه لنخيل البلح معملياً
260 _aCairo :
_bMaha Rashed Ahmed Alsayed ,
_c2018
300 _a81 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Pomology
520 _aThis study was conducted to investigate the ability to produce whole plantlets from immature female inflorescences of date palm trees (Amhat cv.)through tissueCulturestechniq. The best significant result of sterilization that achieved the highest alivespathes was obtained on 30th January and double sterilization with clorex at 50% then 40% each for 30 minute. MS medium supplemented with 10 mg 2,4-D + 3mg 2 ip + 1 mg PBZ resulted in the highest flowery bud elongation . Meanwhile the same medium MS contained PBZ at 2 mg gave a positive result for percentage swelling ,browning and callus, As for production direct somatic embryo, MS medium with added TDZ at 1 mg/L + 0.5 mg/L NAA gave the best result beside the lowest browning percentage. The nitrogen sources NH4 NO3 and KNO3 at 0.83 : 1.43 g/L results in the lowest browning and vitrification degree of friable callus explants. Moreover, this ratio produced the highest globularization degree as well as the highest value of germination percentage and growth rate. Also adding glutamine at 400 mg/L enhanced germination and growth rate of friable callus. MS medium at {u00BD} strength and supplemented with 40 mg/L sucrose produced the highest germination percentage of somatic embryo. MS medium with added 5g/L PEG produced plantlets with high length , root number and root length. After second subculture PEG at 8 g/L produced the highest number of secondary roots as well as it gave the highest percentage survival at acclimatization stage
530 _aIssued also as CD
653 4 _aBA
653 4 _ainflorescences
653 4 _aPalmaceae
700 0 _aEman Mohamed Mostafa Zayed ,
_eSupervior
700 0 _aMohamed Ahmed Fayek ,
_eSupervior
700 0 _aMohamed Helmy Abdelzaher ,
_eSupervior
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aRania
_eCataloger
942 _2ddc
_cTH
999 _c71860
_d71860