| 000 | 02810cam a2200349 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032343.0 | ||
| 008 | 190819s2019 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.07.10.M.Sc.2019.Sa.M | ||
| 100 | 0 | _aSaid Elhussein Said Mahmoud Abuhamed | |
| 245 | 1 | 0 |
_aMolecular studies on oocyte maturation in egyptian buffalo using different media / _cSaid Elhussein Said Mahmoud Abuhamed ; Supervised Salah Eldin Sayed Mohamed Elassal , Ahmed Yehia Gad , Swiefy Abdelrahim Swiefy |
| 246 | 1 | 5 | _aدراسات جزيئية على انضاج بويضات الجاموس المصرى باستخدام بيئات مختلفة |
| 260 |
_aCairo : _bSaid Elhussein Said Mahmoud Abuhamed , _c2019 |
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| 300 |
_a97 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics | ||
| 520 | _aRetinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulusoocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes (PRDX1, SOD1, CAT, HOMX1, and GPX4) using RT-qPCR. (iv) expression level of genes related to oocyte quality (GDF9,BMP15) Maturation rate was significantly improved in 5 nM 9-cisRA oocyte group (95.8%, P < .05) compared to control and other treatment groups (86.7% in control group). The same oocyte group exhibited significantly higher mitochondrial membrane potential activity and lower ROS accumulation level compared to other treatment groups. Antioxidant-related genes were up-regulated in oocytes matured with 5 or 50 nM 9-cisRA compared to control and 200 nM 9-cisRA groups. In contrast, 200 nM of 9-cisRA showed a clear down-regulation for antioxidant-related genes except for PRDX1 | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aAntioxidant genes | |
| 653 | 4 | _aCleavage rate | |
| 653 | 4 | _aFollicular fluid | |
| 700 | 0 |
_aAhmed Yehia Gad , _eSupervisor |
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| 700 | 0 |
_aSalah Eldin Sayed Mohamed Elassal , _eSupervisor |
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| 700 | 0 |
_aSwiefy Abdelrahim Swiefy , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aShimaa _eCataloger |
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_2ddc _cTH |
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_c73401 _d73401 |
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