000			02904cam a2200337 a 4500
003			EG-GiCUC
005			20250223032356.0
008			190911s2018 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.06.M.Sc.2018.El.C
100	0		_aElhussein Mohamed Fouad Mourad Hussein Ahmed
245	1	0	_aCommunity analysis of rhizobacteria associated to plants of deserts and nile using culture dependent and culture independent methods / _cElhussein Mohamed Fouad Mourad Hussein Ahmed ; Supervised Nabil Abrahim Hegazi , Mohamed Fayez Fouad
246	1	5	_aدراسة المجتمع البكتيري المصاحب لجذور النباتات النامية في الاوساط البيئية الصحراوية و وادي النيل باستخدام الطرق المزرعية وتقنيات البيولوجيا الجزيئية
260			_aCairo : _bElhussein Mohamed Fouad Mourad Hussein Ahmed , _c2018
300			_a71 P. : _bcharts ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Microbiology
520			_aIn order to improve the culturability and biomass production of rhizobacteria, we previously introduced plant-only-based culture media to replace chemically-synthetic ones. We herein attempted to widen the scope of plant materials suitable for the preparation of plant-only-based culture media. We chemically analyzed the refuses of turfgrass, cactus, and clover. They were sufficiently rich to support good in vitro growth of rhizobacteria isolates representing Proteobacteria and Firmicutes. They were also adequate and efficient to produce a cell biomass in liquid batch cultures. These culture media were as sufficient as artificial culture media for the cultivation and recovery of the in situ rhizobacteria of barley (Hordeum murinum L.). Based on culture-dependent (CFU plate counting) and culture-independent analyses (qPCR), mowed turfgrass, in particular, supported the highest culturable population of barley endophytes, representing >16% of the total bacterial number quantified with qPCR. This accurately reflected the endophytic community composition, in terms of diversity indices (S{u2019}, H{u2019}, and D{u2019}) based on PCR-DGGE, and clustered the plant culture media together with the qPCR root populations away from the artificial culture media. Despite the promiscuous nature of the plant materials tested to culture the plant microbiome
530			_aIssued also as CD
653		4	_aMicrocolonies
653		4	_aPlant microbiome
653		4	_aPlant-only-based culture media
700	0		_aMohamed Fayez Fouad , _eSupervisor
700	0		_aNabil Abrahim Hegazi , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c73818 _d73818