000			020070000a22003370004500
003			EG-GICUC
005			20250223032358.0
008			061012s2006 ua a f m 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC
041	0		_aEng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.02.M.Sc.2006.TaM.
100	0		_aTawfik Ghiyas Allbabidi
245	1	0	_aMolecular studies on industrilly important , phenylalanine free casein gene in yeast / _cTawfik Ghiyas Allbabidi ; Supervised Sayed Kamel Goda
246	1	5	_aدراسات جزيئية صناعية هامة على الكازين الخالى من الفنيل النين فى الخميرة
260			_aCairo : _bTawfik Ghiyas Allbabidi , _c2006
300			_a74L : _bill ; _c30cm
502			_aThesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry
520			_aAs a prelude to developing a yeast - based fermentation process for the production of phenylalanine free alpha - casein as a foodstuff for patients suffering from phenylketonuria , we cloned a gene encoding a nutritional protein (bovine alpha - casein) , but devoid of phenylalanine codons and with a codon bias similar to that of naturally occurring highly expressed genes in ScerevisiaeThe results show that synthetic gene is expressed in Ecoli when cloned into the Ecoli expression vector pET28a , and expressed in Scerevisiae when cloned into yeast expression vector pYES2 , Western blot analysis has confirmed the expression of the gene understudy conclusion : We cloned and expressed a gene devoid of any phenylalanine codon to produce a foodstuff for PKU patients
530			_aIssued also as CD
653		4	_aphenylalanine free protein
653		4	_aPhenylketonuria
653		4	_aPKU) expression in yeast
653		4	_asynthetic gene expression
700	0		_aSayed Kamel Goda , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAml _eCataloger
905			_aEsam _eRevisor
942			_2ddc _cTH
999			_c73888 _d73888