000			03483cam a2200349 a 4500
003			EG-GiCUC
005			20250223032406.0
008			190925s2019 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.10.M.Sc.2019.Ah.G
100	0		_aAhmed Mahmoud Haggag Abdelazim
245	1	0	_aGenetic studies on some fruit plants / _cAhmed Mahmoud Haggag Abdelazim ; Supervised Ebtissam Hussein Aly Hussein , Basita Abbass Hussein , Neveen Abdelfatah Hassan
246	1	5	_aدراسات وراثية على بعض نباتات الفاكهة
260			_aCairo : _bAhmed Mahmoud Haggag Abdelazim , _c2019
300			_a97 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Genetics
520			_aIn the present study, a reliable protocol for micropropagation of the two Egyptian grapevine (Vitis vinifera L.) cultivars 'Red Romy' and 'Ghariby' was developed using axillary bud explants. Nodal segments were cultured on MS-media (full strength MS or half strength MS) plus 3.0% sucrose and 0.7% agar with four concentrations of BA ,i.e. 0.0, 0.5, 1.0 or 2.0 mg/l for culture establishment. Half strength MS medium with 0.5 mg/l BA showed the highest efficiency in mean shoot length (11.65 and 8.37 cm) and mean number of shoots per explant (3.3 and 3.6) for cultivars 'Red Romy' and 'Ghاariby', respectively. For multiplication, shoots developed from the establishment stage were divided into sections (1.5 - 2 cm in length) and cultured on media comprised of {u00BD}MS basal medium plus 3.0% sucrose and 0.7% agar supplemented with different BA concentrations, i.e. 0.0, 0.5, 1.0 or 2 mg/l alone or in combination with different concentrations of IAA (0.1, 0.3 or 0.5 mg/l). The highest significant shoot length (13.32 and 9.11 cm) was found with growth regulator-free medium for 'Red Romy' and 'Ghariby', respectively. While, the highest number of shoots/explant (4.6 and 3.3) was recorded on {u00BD}MS medium with (2.0 mg/l BA + 0.3 mg/l IAA) and (0.5 mg/l BA + 0.1 mg/l IAA) for 'Red Romy' and 'Ghariby', respectively. Two cultivars ('Red Romy' and 'Ghariby') and a Chinese cultivar ('Cabernet Sauvignon') were successfully cryopreserved by droplet vitrification. Axillary shoot tips were excised from two months old plantlets cultured on solidified {u00BD}MS medium with 0.5 mg/l BA, 3.0% sucrose and 0.7% agar (pH 5.8) at 25 {u25E6}C, under a 12 h light/12 h dark photoperiod with a light intensity of 40 oE m{u2013}2 s{u2013}1. For vitrification, excised shoot tips were precultured on {u00BD}MS solidified medium supplemented with 0.1 M sucrose for 3 days in darkness and then treated with a mixture of 2 M glycerol and 0.4 M sucrose (LS solution) for 20 min at 25 {u25E6}C. Shoot tips were then dehydrated with half-strength PVS2 vitrification solution (30% (w/v) glycerol, 15% (w/v) ethylene glycol, 15 % dimethylsulfoxide and 0.4 M sucrose in MS basal medium, for 30 min
530			_aIssued also as CD
653		4	_aCryopreservation
653		4	_aGrapevine
653		4	_aMicropropagation
700	0		_aBasita Abbass Hussein , _eSupervisor
700	0		_aEbtissam Hussein Aly Hussein , _eSupervisor
700	0		_aNeveen Abdelfatah Hassan , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c74134 _d74134