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003			EG-GiCUC
005			20250223032525.0
008			200220s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.25.M.Sc.2020.Wa.I
100	0		_aWarda Alaa Eldin Mahmoud Hashim
245	1	0	_aIsolation, molecular characterization and expression of nitrite reductase (NiR) gene responsible for the absorption of some air pollutants / _cWarda Alaa Eldin Mahmoud Hashim ; Supervised Said Abdrahman Salih , Rania Mahmoud Abou Ali
246	1	5	_aعزل و توصيف جزيئى والتعبير عن جين مختزل النيتريت المسؤول عن امتصاص بعض ملوثات الهواء
260			_aCairo : _bWarda Alaa Eldin Mahmoud Hashim , _c2020
300			_a86 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology
520			_aA nitrite reductase (NiR) gene was isolated, cloned and sequenced from nitrate induced leaves of the Egyptian Spinacia oleracea seedlings. The sequence of the isolated NiR gene has 1788 bp open reading frame. The NiR sequence was submitted into GenBank under accession number MH729808. The deduced amino acid sequence is 595 a.a, it has 96% homology with predicted ferredoxin-nitrite reductase, chloroplastic (Spinacia oleracea) and highly conserved. Leucine was the highest amino acid percentage in the predicted NiR protein, which has a higher preference for the Ü-helix. RT-PCR analysis showed that the expression level of NiR was the highest at 2 hr of nitrate treatment. The NiR protein had high activity towards NaNO2 substrate after 3 hr induction with potassium nitrate
530			_aIssued also as CD
653		4	_aNitrate treatment
653		4	_aNitrite reductase (NiR) activity
653		4	_aReal Time-PCR
700	0		_aRania Mahmoud Abou Ali , _eSupervisor
700	0		_aSaid Abdrahman Salih , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAsmaa _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c76717 _d76717