000 03413cam a2200349 a 4500
003 EG-GiCUC
005 20250223032528.0
008 200224s2019 ua do f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.09.03.M.Sc.2019.La.M
100 0 _aLaila Akmal Emad Eldien Elokaly
245 1 0 _aMicrobiological assessment after chemo-mechanical caries removal using papain-based enzyme versus conventional rotary tools in occlusal carious lesions :
_bRandomized clinical trial /
_cLaila Akmal Emad Eldien Elokaly ; Supervised Mai Mahmoud Yousry , Essam Abdelhafez Naguib , Mai Mamdouh Mohamed
246 1 5 _aالتقييم الميكروبيولوجى بعد الإزالة الكيميائية للتسوس باستخدام إنزيم بابين مقابل الأدوات الدوارة المعتادة في آفات التسوس الطبقى :
_bدراسة إكلينيكية عشوائية
260 _aCairo :
_bLaila Akmal Emad Eldien Elokaly ,
_c2019
300 _a80 P. :
_bcharts , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Operative Dentistry
520 _aThe study was performed to assess the efficacy of caries excavation using the papain-based chemo-mechanical method (Brix 3000) in comparison to conventional rotary tools in the reduction of Streptococcus Mutans count in Occlusal carious cavities. Materials and methods: Double-blinded, two-armed, split-mouth and a randomized clinical trial was conducted in the clinic of the Conservative Dentistry Department, Faculty of Dentistry, Cairo University, Egypt. Twenty-three patients aged between 18-40 years with active carious lesions in a permanent lower molar (each patient had at least two cavitied molars) were randomly allocated into two groups: a test group (N=23): Brix3000 and a Control group (N=23): Conventional rotary. From each group, two dentin samples were collected before and after caries removal. In both groups, the central cariogenic biomass and superficial parts of the necrotic dentin was removed with the excavator and then discarded. Then, a dentin sample was collected using a sharp, sterile excavator. And then the dentin samples were immediately transferred to the sterile disposable test tube containing 1.5 ml thioglycollate medium used as a carrier and kept in an icebox then taken to the microbiology laboratory for processing within two hours. The sterilized bottles containing the dentin samples were shaken in a vortex for the 30s to disperse bacterial aggregates and decimal dilutions were then prepared in sterile saline (0.9% NaCl). Next, 50 ol aliquots of each dilution were spread using micropipette onto the following solid media and spread on the surface of the agar using a sterile glass rod to give homogenous bacterial growth. Mitis salivarius agar (MSA) was used for Streptococcus spp
530 _aIssued also as CD
653 4 _aChemo-mechanical caries removal
653 4 _aOcclusal carious lesions
653 4 _aPapain-based enzyme
700 0 _aEssam Abdelhafez Naguib ,
_eSupervisor
700 0 _aMai Mahmoud Yousry ,
_eSupervisor
700 0 _aMai Mamdouh Mohamed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAsmaa
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c76787
_d76787