| 000 | 02944cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032534.0 | ||
| 008 | 200321s2019 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.10.10.Ph.D.2019.Su.S | ||
| 100 | 0 | _aSusan Abdelnafea Ismail | |
| 245 | 1 | 0 |
_aSpoligotyping with pncA sequencing strategy conferring the transmission of multidrug{u2011}resistant tuberculosis in Egypt / _cSusan Abdelnafea Ismail ; Supervised Khaled Farouk Mohammed Elamry , Salah Eldin Abdelkarim Selim , Mohamed Galal Aggour |
| 246 | 1 | 5 | _aاستراتيجيه تصنيف سبوليجو مع تتابع بى ان سى ايه للتعرف على انتقال السل متعدد المقاومه للعقاقير فى مصر |
| 260 |
_aCairo : _bSusan Abdelnafea Ismail , _c2019 |
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| 300 |
_a92 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology | ||
| 520 | _aThis study explored the genetic diversity of Mycobacterium tuberculosis isolates in Egypt by spoligotyping in combination with pncA gene sequencing, spoNC. Firstly, 50 isolates out of 400 isolates positive for Mycobacterium tuberculosis were referred to Central Labs Ministry of health were subjected to the analyses, 21 isolates were found to be multidrug resistant (MDR) and 29 isoletes were sensitive for isonizide(INH) and rifampicine(RIF) after testing by phenotypic drug susceptibility testing (DST) and MGIT.Spoligotyping yielded 45 patterns belonging to 7 families that were previously reported in neighboring countries as Iraq, Syria, Iran and Turky. while 4 isolates were orphans. Application of spoNC on obtained Spoligotype patterns enhance to reduce the clustering rate. Bejing family the predominant (34%)were subdivided by pncA sequence into 3 sensitive DST pncA wild type , 3 MDR-DST isolates showing cys14Arg mutation in pncA, 2 sensitive DST isolates with pncA Gly97Asp mutation, and 3 sensitive DST pncAVal128Gly mutation.The next most common CASI_DELHI family (16%) were subdivided by pncA sequencing into CASI_DELHI (st 381, MDR) including 2 pncA silent mutation ser65ser (tcc>tct) and CASI_DELHI (st26, sensitive) which included 6 pncA (WT) results, LAM6 family(6%) all had PncA Gly97Asp mutation, We concluded that SpoNC providesgood snap shot for MDR surveillance and its country origin, and performing early identification of outbreaks in Egyp | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aEgypt | |
| 653 | 4 | _aMycobcterium | |
| 653 | 4 | _aSpoNC | |
| 700 | 0 |
_aKhaled Farouk Mohammed Elamry , _eSupervisor |
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| 700 | 0 |
_aMohamed Galal Aggour , _eSupervisor |
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| 700 | 0 |
_aSalah Eldin Abdelkarim Selim , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c77005 _d77005 |
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