000			03194cam a2200349 a 4500
003			EG-GiCUC
005			20250223032550.0
008			200831s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2020.Gh.I
100	0		_aGhada Mohamed Abdelsalam Ahmed
245	1	0	_aIsolation, identification, biochemical and nanotechnological control of pseudomonas aeruginosa isolated from different patients / _cGhada Mohamed Abdelsalam Ahmed ; Supervised Nagwa Abdel Hamed Tharwat , Rasha Hamed Elshreif , Sherif. M. Elnagdy
246	1	5	_aعزل وتعريف والمقاومة الكيميائيه الحيويه والنانوتكنولوجيه للبسيدوموناس اريجنوزا المعزوله من مختلف المرضى
260			_aCairo : _bGhada Mohamed Abdelsalam Ahmed , _c2020
300			_a139 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology
520			_aPseudomonas aeruginosa was the most frightening pathogen that emergence in health facilities with increasing antibiotic resistance. Nanoparticles have reported as non-antibiotic therapeutic agents that are highly effective in the treatment of P. aeruginosa infections.Combination therapy of nanoparticles and antibiotics considered an alternative therapeutic approach for restoring antibiotic activity.To achieve that goal, we used the synthetic nanoparticles in a combination with ciprofloxacin against MDR P.aeruginosa. The Fifty bacterial isolates were collected from different infection sites the majority of samples was pus samples 46%, followed by sputum samples 22%, followed by urine samples 14%, however, blood culture 6%, pleural fluid, ascitic fluid, and Central Venous Line 4% were recorded as lowest infection sites for P.aeruginosa. Fifty bacterial isolates were further confirmed by traditional PCR as P. aeruginosa by detection of algD gene and Exotoxin A genes. Antibiotic sensitivity test of 50 P. aeruginosa strains was assessed by single disk-diffusion method by using 14 different commercial antibiotics discs. The high resistance rate in strains was recorded against ceftazidime (CAZ) 96%, cefotaxime (CTX) 92%, while the resistance rate exceeds 40% for both Ciprofloxacin (CIP) and Levofloxacin (LEV) (48%). On the other hand, Colistin (CT) showed a higher inhibitory effect (100%) against 50 clinical P. aeruginosa strains while Polymyxin (PB) recorded 98% inhibitory effect against 49 P. aeruginosa strains. Based on antimicrobial susceptibility test, twenty-four MDR P. aeruginosa resistant to ciprofloxacin subsequently was selected for further study
530			_aIssued also as CD
653		4	_aCiprofloxacin (CIP)
653		4	_aLevofloxacin (LEV)
653		4	_aPseudomonas aeruginosa
700	0		_aNagwa Abdelhamed Tharwat , _eSupervisor
700	0		_aRasha Hamed Elshreif , _eSupervisor
700	0		_aSherif. M. Elnagdy , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c77470 _d77470