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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.25.M.Sc.2020.Is.S
100 0 _aIsmail Mohamed Rafeky Hussien Saad
245 1 0 _aStudying HCV IRES sequence and structure for genotype 4a-in correlation to interferon ribavirin responding treatment in Egyptian patients using bioinformatics /
_cIsmail Mohamed Rafeky Hussien Saad ; Supertvised Fawzy Ali Ataby , Salwa Farouk Sabet , Mohamed Ali Saber
246 1 5 _aدراسة المنطقه المسؤله عن الدخول الي الريبوسوم الداخلى فى فيروس التهاب الكبدى سى من حيث التسلسل النيكولتيدى و التركيب الثانوى للسلاله ( 4أ) و علاقه ذلك بالاستجابه لعلاج الانترفيرون\ريبافيرين فى حالات المرضى المصرين باستخدام المعلوماتية الحيوية
260 _aCairo :
_bIsmail Mohamed Rafeky Hussien Saad ,
_c2020
300 _a113 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology
520 _aHepatitis C virus infection is the major cause of chronic hepatitis. The HCV genotype 4a is predominant in Egyptian patients that are not responding to the combined pegylated interferon-alpha /ribavirin therapy. The HCV IRES is a well-defined structure of about 341 nucleotides in its 5{u00B4}-untranslated region (UTR). This study was conducted on 46 Quantification of HCV-RNA in serum has been carried out at the beginning of treatment (W0) and at week12 (W12), patients 26 responders and 20 non-responders to Study HCV IRES Sequence and Secondary Structure and find the Correlation to pegylated Interferon\Ribavirin Responding Treatment in Egyptian patients. There was no statistically significant difference between Responders and Non-Responders regarding gender (P=0.1), age (p=0.3), AFP (p=0.4) and Albumin (p=0.1) while There was a significant increase for AST/ALT (p=0.002, p=0.001) respectively. HCV IRES RT-PCR and sequence analysis including genotyping, MSA and Sequence variation showed that 98% genotype 4a and there was no significant difference in sequence between Responders and Non-Responders patients in addition to the prediction of HCV IRES secondary structure MFE,Centroid revealed that there was no effect on IRES secondary structure and it is conserved among all HCV genotypes
530 _aIssued also as CD
653 4 _aGenotype 4a
653 4 _aHepatitis C Virus (HCV)
653 4 _aPolymerase Chain Reaction (qPCR)
700 0 _aFawzy Ali Ataby ,
_eSupervisor
700 0 _aMohamed Ali Saber ,
_eSupervisor
700 0 _aSalwa Farouk Sabet ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c77636
_d77636