000			03063cam a2200337 a 4500
003			EG-GiCUC
005			20250223032601.0
008			200922s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.03.Ph.D.2020.Da.u
100	0		_aDalia Abdelrahman Ahmed
245	1	0	_aUltra- structure, transcriptional profile and embryonic development of Egyptian buffalo oocytes matured in vitro under oxidative stress / _cDalia Abdelrahman Ahmed ; Supervised Nasser Ghanem Osman , Sherif Mohamed Dessouki
246	1	5	_aالتركيب الخلوى الدقيق: التعبير الجينى وتطور الأجنة لبويضات الجاموس المصرى الناضجة معمليًا تحت ظروف الإجهاد التأكسدى
260			_aCairo : _bDalia Abdelrahman Ahmed , _c2020
300			_a137 P. : _bcharts , facsmilies ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Animal Production
520			_aThis study was performed to investigate cellular and molecular changes of buffalo cumulus-oocyte complexes (COCs) cultured under oxidative stress, and to assess developmental competence after maturation through, in vitro fertilization and early embryonic development up to the blastocyst stage. Morphologically good quality COCs (n=2672) were screened with brilliant cresy- blue (BCB) staining and then graded into three groups (BCB+, BCB - and control). Oocytes from the different groups were evaluated under low (5%) and high oxygen level (20 %), The progress of the nuclear maturation stage and the rate of cumulus expansion were assessed after 24 hrs of in vitro maturation. Ultra-structure of oocytes have been monitored using transmission electron microscope.The fluorescent intensity of lipid, mitochondria, and reactive oxygen species (ROS) were measured using different molecular probes. Transcript abundance of genes regulating different molecular pathways during oocyte maturation was profiled using Real-time PCR. The cumulus expansion of all oocyte groups under low oxygen tension (5%) was higher (P < 0.05) than those cultured under high oxygen tension (20%). Under high oxygen tension, low-competent oocytes (BCB -) showed the lowest maturation rate (P < 0.05). This was associated with an increase in ROS, which was against the BCB+ oocyte pattern. Ultra-structure examination indicated that under low oxygen tension the competent BCB+ oocytes had a higher rate of migration of cortical granules than BCB -. In parallel, the MAPK14 and CPT2 gene expression profile (P < 0.05) was increased under low compared to high oxygen tension
530			_aIssued also as CD
653		4	_aBCB
653		4	_aOocytes
653		4	_aOxidative stress
700	0		_aNasser Ghanem Osman , _eSupervisor
700	0		_aSherif Mohamed Dessouki , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c77859 _d77859