000			03062cam a2200337 a 4500
003			EG-GiCUC
005			20250223032605.0
008			200929s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.08.06.Ph.D.2020.Ha.O
100	0		_aHanzada Tawfik Mohamed Nour Eldin
245	1	0	_aOptimization of the production and purification of lysostaphin and its application to fight staphylococcal biofilms / _cHanzada Tawfik Mohamed Nour Eldin ; Supervised Manal Mohamed Maher , Ahmed Sherif Attia ,
246	1	5	_aتحس{u٠٦أأ}ن إنتاج وتنق{u٠٦أأ}ة الل{u٠٦أأ}زوستاف{u٠٦أأ}ن وتطب{u٠٦أأ}قاته لمكافحة العنقود{u٠٦أأ}ات فى الأغش{u٠٦أأ}ة الح{u٠٦أأ}و{u٠٦أأ}ة
260			_aCairo : _bHanzada Tawfik Mohamed Nour Eldin , _c2020
300			_a149 P. : _bcharts , facsmilies ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520			_aLysostaphin (LST) is an extracellular zinc metalloproteinase bacteriocin secreted by Staphylococcus simulans biovar staphylolyticus that destroys staphylococcal cell wall through its endopeptidase activity. S. aureus imposes a threatening public health issue;hence, interest is raised back in LST.However, its usage is limited due to the lack of affordable, highly-active preparations. Herein the main objective was directed towards achieving sustainable and low-cost LST production platform, using both recombinant and native lysostaphin,to be used in combating S.aureus infections. Recombinant DNA technology was used to clone, express, and purify LST as an N-terminal His-tagged protein in Escherichia coli. A full factorial design was developed in which induction was optimized, being reflected in minimal cost and time. The produced rLST was evaluated in combating biofilms using crystal-violet based assays. This work was further expanded by aiming to develop a novel rLST topical formula targeting staphylococcal skin infections and assessing it in a murine skin model of infection. The rLST optimized production process yielded up to 50 mg/L culture, which is higher than previously reported systems.The purified rLST demonstrated superlative activity in inhibiting staphylococcal biofilms formation as well as detachment of previously established ones, compared to vancomycin and linezolid. Finally, phase inversion composition (PIC) method was successful in incorporating LST in a nano-emulgel (LNEG) which was able to extend active LST release up to 8 h and cure skin infections in a murine skin model
530			_aIssued also as CD
653		4	_aFactorial-design
653		4	_alysostaphin
653		4	_aSustainable
700	0		_aAhmed Sherif Attia , _eSupervisor
700	0		_aManal Mohamed Maher , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c77964 _d77964