000			03224cam a2200349 a 4500
003			EG-GiCUC
005			20250223032617.0
008			201020s2020 ua h f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.10.Ph.D.2020.En.I
100	0		_aEnas Atef Shedeed Younis
245	1	0	_aImmunological studies on salmonellae isolated from different sources / _cEnas Atef Shedeed Younis ; Supervised Jakeen Kamal Abdelhaleem Elgakee , Mahmoud Dardiri Elhariri , Soad Abdelaziz Abdelwanis
246	1	5	_aدراسات مناعيه على السالمونيلا المعزولة من مصادر مختلفة
260			_aCairo : _bEnas Atef Shedeed Younis , _c2020
300			_a76 P. : _bfacsmilies ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aSalmonella infection is a critical veterinary and medical problem world-wide and is a major issue in the food industry. Non-typhoidal Salmonella is known significantly as an important gastroenteritis-related pathogen. There is limited data specifically discussing the outer membrane proteins (OMPs), a distinguishable characteristic of Gram negative bacteria situated at host{u2013} bacterial interface and are significant for virulence, host immune responses and drug therapy targets. Enhanced diagnosis of live poultry colonized with Salmonella species is required to avoid food borne diseases. The widely available ELISA assays are currently based on O-antigens (LPS) mixture or Salmonella total cell lysate and are unorganized by cross-reaction. The present study is based on molecular characterization of OMPs among four Salmonella serovars (S.Typhimurium, S. Enteritidis, S. Kentucky and S. Anatum) using SDS-PAGE, the isolates were confirmed by culturing, biochemical, serological and molecular (real-time PCR targeting invA gene) identifications. The OMPs profiling showed more than 70 protein bands ranging in size from 208 kDa to below 16 kDa which were detected using Totallab 1D 12.2software. It is clear that all Salmonella strains had bands at 54-60 kDa, 45-53 kDa, 36-39 kDa and 26-31 kDa. Eleven strains had bands at 41-46 kDa and 33-35 kDa. Nine strains had bands at 61-69 kDa. Eight strains had bands at 135-145 kDa and 72-79 kDa. Seven strains had bands at 108-123 kDa and 83-91 kDa. In the Western blot analysis, the prepared hyper immune serum of each Salmonella serovars reacted with the outer membrane protein band 35kDa. The finding highlighted that the OMP 35kDa protein of studied salmonellae could be immune-response protein. An Indirect ELISA developed in this study holds promise as screening of poultry flocks for Salmonella infection
530			_aIssued also as CD
653		4	_aELISA
653		4	_aOuter Membrane Proteins
653		4	_aSalmonella
700	0		_aJakeen Kamal Abdelhaleem Elgakee , _eSupervisor
700	0		_aMahmoud Dardiri Elhariri , _eSupervisor
700	0		_aSoad Abdelaziz Abdelwanis , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c78362 _d78362