000 02730cam a2200349 a 4500
003 EG-GiCUC
005 20250223032624.0
008 201105s2020 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.05.M.Sc.2020.Me.M
100 0 _aMenarva Mohamed Moustafa
245 1 0 _aMolecular detection of virulence factors and quinolone resistance determinants in uropathogenic Escherichia coli /
_cMenarva Mohamed Moustafa ; Supervised Zeinat Kamel Mohamed , Doaa Mohamed Ghaith , Mohamed Gamal Farahat
246 1 5 _aالكشف الجزيئى عن عوامل الضراوة و محددات مقاومة الكينولون فى الايشيريشيا كولاى الممرضة للمسالك البولية
260 _aCairo :
_bMenarva Mohamed Moustafa ,
_c2020
300 _a103 P. :
_bcharts , facsmilies ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology
520 _aUropathogenic Escherichia coli is one of the major pathogens causing urinary tract infections. This study aimed to identify the prevalence of antibiotic resistance among pathogenic E. coli isolated from patients with urinary tract infections and evaluation of plant- derived essential oils as alternatives to antibiotics. Of 100 strains, 78 were classified as multidrug resistant. Fifty quinolone-resistant strains were selected and subjected to phenotypic characterization. Furthermore, virulence genes and quinolone-resistance genes were investigated. The most prevalent virulence gene was found to be ear, and results revealed high frequency mutations in quinolone resistance determining region (QRDR) of DNA gyrase (gyrA) and topoisomerase IV (parC) genes. Also, aac(6{u2032})-Ib-cr gene was the most prevalent plasmid-mediated quinolone resistance determinant. The antibacterial potential of ten essential oils was investigated and results revealed that clove and oregano were the most powerful essential oils with MIC 0.25 and 1 æL/mL, respectively. The present results suggest that plant-derived EOs may assist in antimicrobial fight, especially those contain high amounts of eugenol and terpenoids (such as clove and oregano) which are active against multidrug-resistant strains
530 _aIssued also as CD
653 4 _aAntimicrobial
653 4 _aQuinolone
653 4 _aUPEC
700 0 _aDoaa Mohamed Ghaith ,
_eSupervisor
700 0 _aMohamed Gamal Farahat ,
_eSupervisor
700 0 _aZeinat Kamel Mohamed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAmira
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c78574
_d78574