000			03231cam a2200349 a 4500
003			EG-GiCUC
005			20250223032626.0
008			201109s2020 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.10.10.Ph.D.2020.Am.D
100	0		_aAmany Abdellattif Mosleh Abdelfatah
245	1	0	_aDevelopment of a real-time multiplex PCR assay for detection of Salmonellae in chicken samples / _cAmany Abdellattif Mosleh Abdelfatah ; Supervised Jakeen Kamal Abdelhalem Eljakee , Sherif Abdelmonem Omar , Amany Ibrahim Elbialy
246	1	5	_aتطويراختبار تفاعل البلمرة المتسلسل الوقتى الحقيقى متعدد للكشف عن السالمونيلا فى عينات الدجاج
260			_aCairo : _bAmany Abdellattif Mosleh Abdelfatah , _c2020
300			_a72 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aSalmonella is the pathogenic agent of salmonellosis, a major cause of enteric illness and typhoid fever. Salmonella Typhimurium infects poultry causing significant losses, serovar 1, 4, [5], 12: i: - is considered a monophasic variant of S. Typhimurium that increasingly important as public health risk. A total of 58 Salmonella isolates were reidentified by slide agglutination test according to White-Kauffmann- Le Minor scheme. A duplex syber green real time PCR was applied for detection of genus Salmonella and S. Typhimurium using 16S rRNA and fliCgenes. All strains harbor 16S rRNA. Twenty one strains harborfliC gene that including S. Typhimurium (12), S. Kentucky ( 6) , S.1,4,[5],12:i:- (1) , S. Lagos (1) and S. Kedougou (1). A duplex Tagman real time -PCR was performed for differentiation between biphasic S. Typhimurium and monophasic variant 1,4,[5],12:i:- using fljB1,2 and fliB / IS200 in the Fli A. B intergenic region. Ten strains out of 12 S. Typhimurium harbor fljB1,2 however strain S1,4,[5],12:i:- don't possess this gene. While 13 strains were positive to fliB / IS200(12 S. Typhimurium and S 1,4,[5],12:i:-). The fliB / IS200 is specific gene for S .Typhimurium (biphasic and monophasic).These results indicate that two strains serologically confirmed to be S. Typhimurium and S.1,4,[5],12:i:- don't possess fljB1,2 and have fliB/ IS200genes are monophasic variants bya duplex Tagman real time -PCR. It was noticed that prolonged subculture and or repeat phase inversion method leads to formation of flakes that in turn cause wrongly serotyping identification. Real time {u2013}PCR is rapid and can be used to identifying and differentiation between biphasic and monophasic S.1, 4, [5], 12: i: - S. Typhimurium
530			_aIssued also as CD
653		4	_aBiphasic and monophasic S.
653		4	_aFljB1,2 gene
653		4	_aTyphimurium
700	0		_aAmany Ibrahim Elbialy , _eSupervisor
700	0		_aJakeen Kamal Abdelhalem Eljakee , _eSupervisor
700	0		_aSherif Abdelmonem Omar , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c78645 _d78645