| 000 | 02691cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032629.0 | ||
| 008 | 201115s2020 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.12.02.Ph.D.2020.Mo.R | ||
| 100 | 0 | _aMohamed Attia Ragheb Erfan | |
| 245 | 1 | 0 |
_aRegulation of doxorubicin chemotherapeutic potential by MiR-520c-3p in hepatocellular carcinoma cells / _cMohamed Attia Ragheb Erfan ; Supervised Mervat Elsayed Mohamed , Emad Mahmoud Ibrahim Elzayat , Abdelhady Ali Abdelwahab |
| 246 | 1 | _aفى خلايا سرطان الكبد (MiR-520c-3p)تنظيم الامكانية العلاجية للدكسوروبيسين عن طريق الحمض الميكروريبوزى النووى | |
| 260 |
_aCairo : _bMohamed Attia Ragheb Erfan , _c2020 |
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| 300 |
_a160 P . : _bcharts , facsmilies ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry | ||
| 520 | _aBackground: Doxorubicin (Dox) is one of the most common drugs used in cancer therapy, including hepatocellular carcinoma (HCC). Drug resistance, is one of chemotherapy{u2019}s significant problems. Emerging studies have shown that microRNAs (miRNAs) could participate in regulating this mechanism. Nevertheless, the impact of miRNAs on HCC chemoresistance is still enigmatic. Objective: Investigating the role of a candidate miRNA in enhancement of anti-tumor effects of Dox against HepG2 cells. Expression profile for liver related miRNAs (384 miRNAs) has been analyzed on HepG2 cells treated with Dox using qRT-PCR. miR- 520c-3p, the most deregulated miRNA, was selected for combination treatment with Dox. Methods: Expression level for LEF1, CDK2, CDH1, VIM, Mcl-1 and p53 was evaluated in miR-520c-3p transfected cells. Cell viability, colony formation, wound healing as well as apoptosis assays have been demonstrated. Furthermore, Mcl-1 protein level was measured using a western blot technique. Results: The present data indicates that miR-520c-3p overexpression could render HepG2 cells chemo-sensitive to Dox through enhancing its suppressive effects on proliferation, migration, and induction of apoptosis | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aChemosensitivity | |
| 653 | 4 | _aHepatocellular carcinoma | |
| 653 | 4 | _amiR-520c-3p | |
| 700 | 0 |
_aAbdelhady Ali Abdelwahab , _eSupervisor |
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| 700 | 0 |
_aEmad Mahmoud Ibrahim Elzayat , _eSupervisor |
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| 700 | 0 |
_aMervat Elsayed Mohamed , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aAmira _eCataloger |
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| 905 |
_aNazla _eRevisor |
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| 942 |
_2ddc _cTH |
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| 999 |
_c78767 _d78767 |
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