000 02762cam a2200349 a 4500
003 EG-GiCUC
005 20250223032642.0
008 201210s2020 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.25.M.Sc.2020.No.R
100 0 _aNoha Ashraf Hassan
245 1 0 _aRadio sensitization effect of 5-fluorouracil modified with zinc oxide nanoparticles on human cell lines /
_cNoha Ashraf Hassan ; Supervised Mohamed Aly Edesouky , Abdellatif Mohamed Salah Eldin , Soheir Saad Korraa
246 1 5 _aتأثير الحساسية الأشعاعية لل 5- فلورويوراسيل المعدل بالجسيمات النانونية لأكسيد الزنك على خطوط الخلايا البشرية
260 _aCairo :
_bNoha Ashraf Hassan ,
_c2020
300 _a164 P. :
_bcharts , facsmilies ;
_c25cm
502 _aThesis (M.Sc.)- Cairo University - Faculty of Science - Department of Biotechnology
520 _aThe aim of the study is to load 5-fluorouracil on zinc oxide nanoparticles that modified with cetrimonium bromide (CTAB) to synthesize the nanocomposite for enhancing the anticancer activity of 5-FU. The in vitro anticancer activity of this nanocomposite was evaluated without and with radiation on three different cancer cell lines (HepG2, HUH- 7 and MCF-7) and one normal cell line (WI-38) by MTT assay after 24 and 48 hr in comparison to 5-FU and nanocomposite without radiation respectively. The expression of apoptotic genes (P 53 and Bax) and antiapoptotic gene (Bcl-2) was investigated by using liver cancer cell line (HepG2) and also the level of lipid peroxidation and antioxident molecules and enzymes was measured by using different treatments whether combined or not with radiation. The results showed that the nanocomposite exerted better anticancer activity against different cancer cells while posing minimal effect on normal cell line after 24 and 48hr in comparison with pure 5-FU. Then nanocomposite with radiation exerted better anticancer activity than pure nanocomposite after 48 hr on HepG2. Nanocomposite not only potentiated the toxic effects of radiation and led to a higher rate of cell death but also, enhanced the increase in apoptotic genes expression and decrease in antiapoptotic genes expression
530 _aIssued also as CD
653 4 _a5-Fluorouracil
653 4 _aCTAB
653 4 _aZinc oxide NPs
700 0 _aAbdellatif Mohamed Salah Eldin ,
_eSupervisor
700 0 _aMohamed Aly Edesouky ,
_eSupervisor
700 0 _aSoheir Saad Korraa ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAmira
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c79166
_d79166