000			02880cam a2200349 a 4500
003			EG-GiCUC
005			20250223032649.0
008			201231s2020 ua dho f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.25.M.Sc.2020.No.C
100	0		_aNorhan Yasser Sayed Moaz
245	1	0	_aCharacterization and functional analysis of an insecticidal protein from baculovirus / _cNorhan Yasser Sayed Moaz ; Supervised Saeed Ahmed Soliman Ghozlan , Maha Mohamed Youssef Alkhazindar , Wael Hassan Ali Elmenofy
246	1	5	_aالتوصيف والتحليل الوظيفي لبروتين مبيد للحشرات من الفيروس العصوي
260			_aCairo : _bNorhan Yasser Sayed Moaz , _c2020
300			_a61 P . , (12) P. : _bcharts , facsmilies , photographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology
520			_aChitinase plays a highly important function in the growth of insects and its development by hydrolyzing chitin in the body of the insect. In this study we have cloned, expressed and characterized chitinase gene A (ChiA) from the cotton leafworm, Spodoptera littoralis nucleopolyhedrovirus, an Egyptian isolate. The Spli-ChiA gene shown to have an open reading frame of 1809 nucleotides, encoding a protein of 599 amino acid residues with a predicted weight of 66.8 kDa. The phylogenetic analysis revealed that the Spli-ChiA deduced protein sequence shared extensive similarities with other baculovirus chitinases to be found in the GenBank. The SDS-PAGE analysis of SpliNPV-infected larvae total protein followed by western blotting with anti-SpliNPV chitinase antibody identified a protein of about ~50 kDa corresponding to the mature chitinase of SpliNPV. Temporal transcriptional analyses using RT-PCR and qRT-PCR revealed that Spli-ChiA transcription might be detected in SpliNPV infect larvae at 12 hpi with a steady increase in the transcription levels up to 71.4-fold at 120 hpi, suggesting the late transcription of chitinase gene. In addition, the purified Spli-ChiA protein showed inhibition in vitro of the growth of two fungi species, Fusarium solani and Fusarium oxysporum, confirming the expressed protein activity. The results concluded that SpliNPV chitinase (Spli-ChiA) is a good applicant protein for significant contribution in pest control
530			_aIssued also as CD
653		4	_aChitinase gene A
653		4	_aqRT-PCR
653		4	_aSpodoptera littoralis NPV
700	0		_aMaha Mohamed Youssef Alkhazindar , _eSupervisor
700	0		_aSaid Ahmed Soliman Ghozlan , _eSupervisor
700	0		_aWael Hassan Ali Elmenofy , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aAmira _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c79396 _d79396