000			03338cam a2200337 a 4500
003			EG-GiCUC
005			20250223032658.0
008			210124s2020 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.12.02.Ph.D.2020.Na.M
100	0		_aNashwa Medhat Ahmed Essa
245	1	0	_aMultidrug resistance (MDR1) gene expression, FMS-Like tyrosine kinase-3 (FLT3-ITD) and nucleophosmin (NPM1) as prognostic biomarkers in acute myeloid leukemic Egyptian patients / _cNashwa Medhat Ahmed Essa ; Supervised Mohamed Ali Eldesouky , Neemat Mohamed Kassem
246	1	5	_aوالنيوكليوفوزمين كنذير للمؤشرات الحيوية فى المرضى المصريين المصابيين بسرطان الدم الميلودي الحاد (NPM 1)كيناز3 (FLT3-ITD )التيروزين (MDR1)دراسة التعبير الجينى لجينات المقاومة المتعددة للأدويه
260			_aCairo : _bNashwa Medhat Ahmed Essa , _c2020
300			_a130 P. : _bcharts , facimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
520			_aBackground: Acute Myeloid Leukemia (AML) is a heterogeneous disorder with variable genetic abnormalities and cytogenetic alterations which provide a significant disease prognosis and determine response to therapy. Purpose: We aim to investigate the expression of the MDR1 gene in 100 Egyptian AML patients, to identify their role on both the progression and chemotherapeutic refractoriness together with assessment of known prognostic molecular markers; FLT3-ITD and NPM1 mutations. Methodology: Quantitative assessment of MDR1 gene expression was performed by quantitative RTPCR. Additional prognostic molecular markers were determined as internal tandem duplications of the FLT 3 gene and nucleophosmin gene mutation A.Results: MDR1 gene expression levels & FLT3/ITD mutations were significantly higher in AML patients with resistant disease with P value <0.001 & 0.002 respectively. However, NPM1 was insignificantly higher in patients with CR P-value 0.14. In MDR positive group, wild FLT3/ITD with or without NPM1 mutation was favorable in achieving CR with p value 0.02. MDR negative group, wild FLT3/ITD with or without NPM1 mutation showed insignificantly higher CR rates with P value (0.35). Kaplan-Meier curves revealed statistically significant difference between MDR1- negative and MDR1-positive patients regarding their DFS and OS between the two groups where DFS & OS were higher in MDR1-negative patients with p value 0.004 & 0.01, respectively. Conclusion: the results obtained by the current work together with the previous researches concerning the study of multidrug resistance genes in AML patients provide additional evidence of the role played by these genes as predictors of chemoresistance and poor treatment outcome
530			_aIssued also as CD
653		4	_aAcute Myeloid Leukemia
653		4	_aChemoresistance
653		4	_aTreatment outcome
700	0		_aMohamed Ali Eldesouky , _eSupervisor
700	0		_aNeemat Mohamed Kassem , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c79670 _d79670