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008 210131s2020 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.02.Ph.D.2020.As.E
100 0 _aAsmaa Kamel Hammad
245 1 0 _aEffect of streptozotocin-induced diabetes mellitus on the cerebellar cortex of adult male albino rat and the curative role of stem cells versus curcumin :
_bHistological, biochemical, immunohistochemical and gene expression Study /
_cAsmaa Kamel Hammad ; Supervised Hafiz Waly Ahmed , Ayman Aboelenein Rizk , Shereen Abdelfattah Mohamed
246 1 5 _aتأثير مرض السكرى المستحث بالاستربتوزوتوسين على قشرة المخيخ فى ذكور الجرذان البيضاء البالغة والتأثير العلاجى للخلايا الجذعية مقارنة بالكركم :
_bدراسة نسيجية كيميائية حيوية: هستوكيميائية مناعية و جينية
260 _aCairo :
_bAsmaa Kamel Hammad ,
_c2020
300 _a125 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Anatomy
520 _aBackground: Diabetes mellitus is the disease, termed either by insulin paucity or resistance and hyperglycemia. The selection of the cerebellum was built on its specific functions.The aim of this study was to investigate a comparison between the possible therapeutic effects of MSCs and curcumin against fluctuations in the cerebellar cortex of STZ-induced diabetic albino rats. Materials and methods: Forty rats were divided into five groups: control, sham control, streptozotocin-induced diabetes, diabetes and MSCs administered and diabetes and curcumin administered. Light microscopic (H&E), immune-histochemical; Glial fibrillary acidic protein (GFAP) and caspase-3, real-time PCR; phospholipase-C (PLC) and Ü-synuclein, histomorphometric analysis, oxidative / anti-oxidatants; malondialdehyde (MDA)/ superoxide dismutase (SOD) glutathione (GSH) and were made. Results: The histopathological examination of the STZ-induced diabetic rats revealed alterations in the molecular, purkinje and granular layers. Abnormal organizations, vacuolation, patchy loss of purkinje cells were detected. Some purkinje cells migrated into the granular layer.Hemorrhage in pia mater outspreading to cerebellar layers is discerned. Purkinje cells showed karyorrhexis.The mean value of area percentage for GFAP and caspase-3 immune- reactivity revealed significant increase compared to that of the control group. Also, MDA level was significantly increased while the SOD and GSH levels were significantly lower when compared to the control group. Meanwhile, mean values of PLC demonstrated significant decrease, while Ü-synuclein levels displayed a significant increment in the diabetic group. Administration of curcumin and MSCs extremely ameliorated the previous alterations.Conclusion: the deleterious alterations on the cerebellar cortex induced by diabetes were obviously improved when treated with either MSCs or curcum
530 _aIssued also as CD
653 4 _aCerebellum
653 4 _aDiabetes mellitus
653 4 _aStem cells
700 0 _aAyman Aboelenein Rizk ,
_eSupervised
700 0 _aHafiz Waly Ahmed ,
_eSupervisor
700 0 _aShereen Abdelfattah Mohamed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c79776
_d79776