000 03152cam a2200349 a 4500
003 EG-GiCUC
005 20250223032705.0
008 210215s2020 ua dhbof m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.07.03.Ph.D.2020.Ma.G
100 0 _aMarwa Mostafa Mohamed Ahmed
245 1 0 _aGenotyping native and exotic rabbit breeds grown in Egypt /
_cMarwa Mostafa Mohamed Ahmed ; Supervised Essam Abbas Elgendy , Shoukry Mohamed Eltantawy , Soha Sayed Abdelmagid
246 1 5 _aالتركيب الجينى لسلالات الأرانب المحلية والأجنبية النامية فى مصر
260 _aCairo :
_bMarwa Mostafa Mohamed Ahmed ,
_c2020
300 _a139 P. :
_bcharts , facimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Animal Production
520 _aThis study aimed at genotyping the native and exotic rabbit breeds in Egypt and determining DNA barcodes of them. Rabbits of seven breeds were used in this experiment. The native breeds were Black Baladi (BB), White Baladi (WB), Red Baladi (RB) and Egyptian Jabali (JAB). The exotic breeds were New Zealand White (NZW), American Rex (AR) and Chinchilla (CH). Genotyping included detecting the microsatellite markers in 14 loci and sequencing the mitochondrial DNA (mtDNA) cytochrome oxidase 1 (CO1). The results revealed breeds varied in the observed number of alleles (No) and the effective number of alleles (Ne). The differences between No and Ne were small in WB (0.2), RB (0.2), AR (0.1) and CH (0.2), and this was possibly due to maintaining the breeds in small size populations. The differences were larger in the breeds BB (2.3), JAB (1.1) and NZW (2.0) and indicated possible inbreeding and/or selection. The observed heterozygosity (Ho) was intermediate to high among the breeds. The average expected heterozygosity (He) was the highest (0.82) in NZW and was the lowest (0.46 and 0.47) in AR and CH. The difference between Ho and He was small (0.01-0.06) in BB, WB, RB, JAB and NZW, and was large in AR (0.26) and CH (0.14). The mean polymorphic information content (PIC) was high in BB, JAB and NZW (PIC {u2265}0.50) and was moderate in WB, RB, AR and CH (PIC {u2264} 0.50). The FIS was negative in most of the loci and indicated the excess in heterozygosity and the decrease in homozygosity. Except JAB, there was overlapping between many breeds indicating possible gene exchange. The results of sequencing mtDNA revealed that the (A+G)/(C+T) ratio was different between breeds and ranged from 0.695 in BB to 0.714 in CH.The nucleotide diversity among breeds resulted in different gene encoding
530 _aIssued also as CD
653 4 _aCOI gene
653 4 _aHaplotypes
653 4 _aMicrosatellite markers
700 0 _aEssam Abbas Elgendy ,
_eSupervisor
700 0 _aShoukry Mohamed Eltantawy ,
_eSupervisor
700 0 _aSoha Sayed Abdelmagid ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c79901
_d79901