000			02936cam a2200349 a 4500
003			EG-GiCUC
005			20250223032720.0
008			210327s2021 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.02.M.Sc.2021.Ay.B
100	0		_aAya Khaled Mahmoud Ateia
245	1	0	_aBiochemical studies on the production of extracellular xylanase from some locally isolated marine bacteria / _cAya Khaled Mahmoud Ateia ; Supervised Ismail Abdelshafy Abdelhamid , Heba Mohamed Kamal , Manal Selim Mohamed
246	1	5	_aدراسات ب{u٠٦أأ}وك{u٠٦أأ}م{u٠٦أأ}ائ{u٠٦أأ}ة لانتاج انز{u٠٦أأ}م الز{u٠٦أأ}لان{u٠٦أأ}ز من بعض انواع بكت{u٠٦أأ}ر{u٠٦أأ}ا البحار المعزولة محل{u٠٦أأ}ا
260			_aCairo : _bAya Khaled Mahmoud Ateia , _c2021
300			_a102 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry
520			_aXylanase is one of the most important enzymes consumed industrially and biotechnologically as hydrolyzing xylan that is widely spread among agro wastes accounting for about one-third of the renewable organic carbon on Earth Xylanases are produced by many organisms. This study was carried out to produce xylanase enzymes from marine bacteria to improve the production and study the effect of the produced enzyme on lignocellulosic agriculture waste to be used in industrial and biotechnological applications. By identification of the selective strain by using 16sRNA, the isolate was a new strain called Bacillus subtilis AKM1 and has the accession number MK377251.The optimum culture conditions for xylanase production from Bacillus subtilis AKM1 were as follows incubation period (48 hr), initial pH (7.0), agitation speed (100 rpm), incubation temperature(40{u00B0}C). After the purification, xylanase showed maximal activity at 50 {u00B0}C and pH 7.0. metal ions as Mn²⁺, Ca²⁺, and Fe²⁺ increased the residual activity by increasing the concentration, while Ag+, SDS, and EDTA completely inhibited the activity at higher concentrations. the molecular weight was 34 KDa , Km 0.044 mM and Vmax 22.222. Xylooligosaccharides produced by enzymatic hydrolysis using the purified xylanase showed antimicrobial activity, antioxidant activity, and anti-tumor activity against breast and colon cancer cells
530			_aIssued also as CD
653		4	_aExtracellular xylanase
653		4	_aXylan and Xylo-oligosaccharide
653		4	_aXylanase
700	0		_aHeba Mohamed Kamal , _eSupervisor
700	0		_aIsmail Abdelshafy Abdelhamid , _eSupervisor
700	0		_aManal Selim Mohamed , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c80383 _d80383