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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.02.Ph.D.2021.Re.E
100 0 _aReda Ibrahim Abdalmohaimen Amer
245 1 4 _aThe effect of mesenchymal stem cells, with and without Melatonin, on the islets of Langerhans of induced diabetic adult male albino rat :
_bHistological, immunohistochemical and fluorescent study /
_cReda Ibrahim Abdalmohaimen Amer ; Supervised Hoda Mahmoud Elaasar , Laila Ahmed Rashed , Abir Oueida Elsadik
246 1 5 _aدراسة هستولوجية: هستوكيميائية مناعية و مستشعة :
_bتأثيرالخلايا الجذعية الوسيطة: مع وبدون الملاتونين: على جزر لانجرهانز فى مرض السكرى المستحث في ذكور الفئران البيضاء البالغة
260 _aCairo :
_bReda Ibrahim Abdalmohaimen Amer ,
_c2021
300 _a118 P . :
_bcharts , facsmilies ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Anatomy
520 _aDiabetes is prevailing everywhere and adversely affecting the lives of individuals.Treatment of diabetes using insulin administration, oral antidiabetic drugs, whole pancreas and islets of Langerhans transplantation faced considerable limitations. Mesenchymal Stem cells (MSCs) inspired medical research with a promising new era in the treatment of DM having an advantage of easy short operation with minimal invasiveness of patients. Objective: To compare the effects of bone marrow MSCs and adipose tissue MSCs on the islets of Langerhans in diabetic rats, detect the enhancing role of melatonin (MT) on MSCs and elicit the mechanisms that induce islet cell regeneration.Material and methods: Sixty adult male albino rats were divided into six equal groups: group I (Control) received nothing, the remaining fifty were given a single dose of STZ of 50 mg/kg intraperitoneally, diagnosed diabetic by blood glucose higher than 220 mg/dl and were divided into: group II (STZ) received nothing, group III received a single intravenous injection of BM-MSCs, group IV received a single intravenous injection of AD-MSCs, group V received a single intravenous injection of MT pretreated BM-MSCs, group VI received a single intravenous injection of MT pretreated AD-MSCs. Results: AD-MSCs were significantly better compared to BM-MSCs in improving body weight, blood glucose, fasting serum insulin, IL-17, IL-10, area percent of PKH labelled MSCs, PCNA positive islets cells nuclei, Caspase-3 immuno-expression, PDX1 gene expression, Glut2 gene expression. MT pretreated MSCs gave significantly better results compared to untreated ones.Conclusion: MSCs could be a promising treatment of DM. MT pretreated AD-MSCs were the best in preservation and regeneration of the islet of Langerhans of the pancreas.
530 _aIssued also as CD
653 4 _aDiabetes
653 4 _aIslets of Langerhans
653 4 _aMesenchymal stem cells
700 0 _aAbir Oueida Elsadik ,
_eSupervisor
700 0 _aHoda Mahmoud Elaasar ,
_eSupervisor
700 0 _aLaila Ahmed Rashed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aAmira
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c80458
_d80458